en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="color:#ffffff;"><strong><span style="background-color:#008080;">Background and Objective</span></strong><span style="background-color:#008080;">:</span></span> Efflux pump inhibitors (EPIs) can block efflux pumps and are helpful in potentiating the activity of aminoglycosides against <em>Pseudomonas aeruginosa</em>. The present study compared the effects of phenylalanine-arginine beta naphthylamide (PA&beta;N) and curcumin on aminoglycoside minimum inhibitory concentration (MIC) on <em>Pseudomonas aeruginosa</em> clinical isolates.<br> <span style="color:#ffffff;"><strong><span style="background-color:#008080;">Materials and Methods</span></strong><span style="background-color:#008080;">:</span></span> For this descriptive-analytical study, 100 clinical isolates of <em>Pseudomonas aeruginosa</em> were collected and identified by differential diagnostic tests. The MICs of amikacin, gentamicin, and tobramycin were evaluated before and after adding EPIs using a micro-broth dilution test.<strong><span dir="RTL"></span></strong><br> <span style="color:#ffffff;"><strong><span style="background-color:#008080;">Results:</span></strong></span> The bacteria were isolated from different types of samples, including urine (26 isolates), sputum (37 isolates), ulcers (20 isolates), catheters (eight isolates), blood (five isolates), feces (two isolates), and eyes (two isolates). Overall, 60% of the isolates were obtained from males (mean age = 47.85), and 40% from females (mean age = 44.76).<br> In the MIC test, 11 (25.5%), 15 (34.8%), and 18 (41.8%) isolates were resistant to amikacin, gentamicin, and tobramycin, respectively. Significant reductions in the MICs of amikacin, gentamicin, and tobramycin were observed after adding curcumin in 54-100% of aminoglycoside-resistant isolates, while fewer changes in the MICs of aminoglycosides were seen against these clinical isolates after adding PA&beta;N (36-55%).<span dir="RTL"></span><br> <span style="color:#ffffff;"><strong><span style="background-color:#008080;">Conclusion</span></strong><span style="background-color:#008080;">:</span></span> Curcumin and PA&beta;N can potentiate the effect of aminoglycosides on clinical isolates of <em>Pseudomonas aeruginosa</em> and change their susceptibility pattern due to efflux pump inhibition. However, our outcomes detected that curcumin was more effective than the PA&beta;N against the aminoglycoside-resistant isolates of <em>P. aeruginosa</em>.</span></span></p> Curcumin, Efflux pump, Inhibitor, Phenylalanine arginine beta naphthylamide, Pseudomonas aeruginosa 105 110 http://journal.zums.ac.ir/browse.php?a_code=A-10-5348-1&slc_lang=en&sid=1 Parisa Charkhi parisacharkhi@yahoo.com 5200319475328460051689 5200319475328460051689 No Molecular and Cell Biology Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Mohammad Reza Haghshenas haghshenas2001@yahoo.com 5200319475328460051690 5200319475328460051690 No Dept. of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Bahman Mirzaei dr.bahman.m@gmail.com 5200319475328460051691 5200319475328460051691 No Dept. of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Lotfollah Davoodi lotfdavoodi@yahoo.com 5200319475328460051692 5200319475328460051692 No Antimicrobial Resistance Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Zahra Norouzi Bazgir zahranorozi53@yahoo.com 5200319475328460051693 5200319475328460051693 No Molecular and Cell Biology Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Hamid Reza Goli goli59@gmail.com 5200319475328460051694 5200319475328460051694 Yes Molecular and Cell Biology Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran