en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="direction: ltr; font-style: normal; text-align: justify;"><span style="display: none;">&nbsp;</span><span style="display: none;">&nbsp;</span><span style="display: none;">&nbsp;</span><span style="font-family:Times New Roman;"><span style="font-size:16px;"><strong><span style="background:#2D7F8F;"><span style="color:white;">&nbsp;Background & Objective:</span></span> </strong>&nbsp;<span style="color: black;">Acetaminophen is known as the most common agent which causes hepatic and renal toxicity in human and experimental animals at supra-therapeutic doses. The current study investigated the protective effects of <em>Descurainia sophia</em> seed extract on the acetaminophen-induced nephrotoxicity markers in male mice.</span><br> <strong><span style="letter-spacing:-.1pt;background:#2D7F8F;"><span style="color:white;">&nbsp;Materials & Methods:</span></span></strong> &nbsp;<span style="letter-spacing: -0.1pt;"><span style="color:black;">Experimental male mice (n=60, Swiss albino mice) were divided into six groups as follows: Group A (control group), Group B (acetaminophen group), and treatment groups including T1, T2, T3, and T4 (<em>D. sophia</em> seed extract groups). Toxicity was induced by acetaminophen (500 mg/kg). The mice administered <em>D. sophia</em> seed extract for 7 days in doses of 50, 100, 200, and 300 mg/kg. In the next step, animals were euthanized 24 hours after acetaminophen administration. Blood samples were collected. Serum levels of blood urea nitrogen (BUN), creatinine, and uric acid were analysed. Furthermore, kidney tissues were removed for histopathological examination via haematoxylin and eosin staining.</span></span><br> <strong><span style="background:#2D7F8F;"><span style="color:white;">&nbsp;Results: </span></span>&nbsp;</strong><span style="letter-spacing: -0.1pt;"><span style="color:black;">Our data revealed that acetaminophen increased the levels of BUN, creatinine and uric acid (<em>P</em><0.05). Pre-treatment of <em>D. sophia</em> seed extract decreased the serum BUN, creatinine and uric acid significantly compared to the acetaminophen group (<em>P</em><0.05). Additionally, in histopathological examination, <em>D. sophia</em> extract had restored acetaminophen-induced nephrotoxicity, particularly in the dose of 300 mg/kg.&nbsp;</span></span></span></span><br> <span style="font-family:Times New Roman;"><span style="font-size:16px;"><strong><span style="background:#2D7F8F;"><span style="color:white;">&nbsp;Conclusion: </span></span></strong>&nbsp;&nbsp;<span style="letter-spacing: -0.1pt;"><span style="color:black;">The present findings suggested that oral administration of D. sophia seed extract has protective effect against acetaminophen nephrotoxicity in mice.</span></span></span></span><span style="display: none;">&nbsp;</span></p> <p style="text-align: justify;"><p style="font-style:normal;"></p><p style="font-style:normal;"></p></p> <p style="text-align: justify;"><p style="font-style:normal;"></p></p> <p></p><p style="font-style:normal;"></p><div style="direction: ltr;"></div><div style="direction: ltr;"></div><p style="font-style:normal;"></p><div style="direction: ltr;"></div> <div style="direction: ltr;"><span style="font-size:14px;"><span style="display: none;">&nbsp;</span></span></div> <div style="direction: ltr;"><strong><span style="display: none;">&nbsp;</span></strong></div> Acetaminophen, BUN, Creatinine, Descurainia sophia seed, Mice, Nephrotoxicity 139 144 http://journal.zums.ac.ir/browse.php?a_code=A-10-2008-1&slc_lang=en&sid=1 Pedram Moshaie-Nezhad pedram.moshaee@gmail.com 5200319475328460056477 5200319475328460056477 No Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Zahra Bahari bahari_441@yahoo.com 5200319475328460056478 5200319475328460056478 No Dept. of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Zohreh Jangravi jangraviz89@gmail.com 5200319475328460056479 5200319475328460056479 No Dept. of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Seyed Mohammad Zarei sm.zarei63@gmail.com 5200319475328460056480 5200319475328460056480 No Dept. of Pharmacognosy, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran Maryam Iman iman1359@yahoo.com 5200319475328460056481 5200319475328460056481 Yes Dept. of Pharmaceutics, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran