en Life Science Life Science مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:16px;"><span style="color:#ffffff;"><strong><span style="background-color:#16a085;">Background and Objective:</span></strong></span> The rate of survival in acute promyelocytic leukemia (APL) can dramatically improve, if the patients receive all-trans-retinoic acid (ATRA) treatment. However, this drug&#39;s toxicity is a major problem in APL treatment. Previous researches have demonstrated that phyto-polyphenols such as epigallocatechin gallate (EGCG) and kaempferol cause apoptosis in hematopoietic neoplasms, but do not negatively impact healthy cells. The present study compared the differentiation effects of kaempferol and EGCG, as well as ATRA in NB4 leukemia cells during five days.<br> <span style="color:#ffffff;"><strong><span style="background-color:#16a085;">Materials and Methods:</span></strong></span> Herein, kaempferol and EGCG&#39;s differentiation-inducing activity was examined by NBT assay and Real-time PCR in leukemia NB4 cells.<br> <span style="color:#ffffff;"><strong><span style="background-color:#16a085;">Results:</span></strong></span> EGCG (25 &micro;M) and kaempferol (50 &micro;M) induced the NB4 cells&#39; differentiation, towards a granulocytic pattern similar to ATRA (1 &mu;M). EGCG further suppressed PML/RAR&alpha; clinical marker&#39;s expression compared to kaempferol, it also decreased HDAC1 expression in leukemia NB4 cells.<br> <span style="color:#ffffff;"><strong><span style="background-color:#16a085;">Conclusion:</span></strong></span>&nbsp;Based on this study, compared to kaempferol, EGCG at low concentrations is preferred for long-term ATRA therapy in APL patients.</span></span></p> Acute promyelocytic leukemia, Epigallocatechin gallate, Kaempferol, Differentiation, HDAC1, PML/RARα 339 345 http://journal.zums.ac.ir/browse.php?a_code=A-10-5601-1&slc_lang=en&sid=1 Mohammad Hassan Jokar jokarmh@mums.ac.ir 5200319475328460088883 5200319475328460088883 No Golestan Rheumatology Research Center, Sayad Shirazi Hospital, Golestan University of Medical Sciences, Gorgan, Iran Sima Sedighi simasedighy@yahoo.com 5200319475328460088884 5200319475328460088884 No Golestan Rheumatology Research Center, Sayad Shirazi Hospital, Golestan University of Medical Sciences, Gorgan, Iran Maliheh Moradzadeh moradzadeh63@yahoo.com 5200319475328460088885 5200319475328460088885 Yes Golestan Rheumatology Research Center, Sayad Shirazi Hospital, Golestan University of Medical Sciences, Gorgan, Iran