Journal of Advances in Medical and Biomedical Research

en Evaluation of Specific Bacteriophage Against Salmonella infantis and Its Antibacterial Effects Compared to Ciprofloxacin in In Vitro Conditions Medical Biology Medical Biology مقاله پژوهشی Original Research Article Background and Objective: Phage therapy could be used as an alternative method to antibiotic treatments. The purpose of this study was to evaluate the antibacterial activities of isolated lytic bacteriophage against ciprofloxacin-resistant strain of Salmonella infanits in vitro conditions. Materials and Methods: The standard strain of Salmonella infantis  and its specific bacteriophage was isolated by soft agar method. Phage susceptibility to heat and pH was evaluated by the Double-Layer Agar method.  In vitro assay was carried out on human epithelial type 2 (HEp-2) cells to investigate the effect of bacteriophage on the cytotoxic and invasion of Salmonella infantis to human epithelial cells. Results: Head and tail morphology of bacteriophages against Salmonella infantis were identified by transmission electron microscopy and assigned to the Myoviridae family. The results of the double-layer agar assay showed that the titer of bacteriophages was 1.8×107 PFU/ml. bacteriophage was stable at 4 ֯C and the best quantification of bacteriophage was determined at pH=8. The isolated bacteriophage was specific for Salmonella infantis and had no lytic activity against other pathogenic bacteria. In the evaluation of the binding and invasion of Salmonella infantis to the HEp-2 cell line, as expected, the lytic activity of specific bacteriophage was observed following inoculation. Conclusion: Additional studies are needed for better understanding of the interaction between phage, microorganisms and human host before applying phage therapy on a large scale. Salmonella, Salmonellosis, Lytic bacteriophage, Ciprofloxacin 154 161 http://journal.zums.ac.ir/browse.php?a_code=A-10-460-3&slc_lang=en&sid=1 Mohammad Reza Esmaeil Zadeh mamazblackrose1367@gmail.com 5200319475328460073411 5200319475328460073411 No Dept. of Pathobiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Kazem Sharifi Yazdi mksharifi@tums.ac.ir 5200319475328460073412 5200319475328460073412 No Zoonosis Research Center, Tehran University of Medical Sciences, Tehran, Iran Zahra Rajabi z_rajabi.arshad@yahoo.com 5200319475328460073413 5200319475328460073413 No Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran Farzaneh Amin Harati shahrood2005@gmail.com 5200319475328460073414 5200319475328460073414 No Dept. of Pathobiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran Farhad Nikkhahi farhadnikkhahi@gmail.com 5200319475328460073415 5200319475328460073415 No Dept. of Pathobiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran Sara Sharifi Yazdi mksharifiyazdi@gmail.com 5200319475328460073416 5200319475328460073416 No Medical Student, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Gholamreza Hassanpour hassanpour@tums.ac.ir 5200319475328460073417 5200319475328460073417 No Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran Alireza Monadi Sefidan 5200319475328460073418 5200319475328460073418 No Dept. of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran Mohammad Mehdi Soltan Dallal msoltandallal@gmail.com 5200319475328460073419 5200319475328460073419 Yes Dept. of Pathobiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran