en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong>The neuroprotective potential of 1,8-Cineole (CIN) has recently been documented in vitro. Here we studied potential beneficial therapeutic effects of CIN, using the temporal lobe epilepsy (TLE) pilocarpine rat model through up-regulation of <em>Bcl-2,</em> as an anti-apoptotic gene.<br> <strong><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span> </strong>A total of 32 (n=8 per group) male Wistar rats were divided into 4 groups as follows:&nbsp; i) normal rats (received CIN (50 mg/kg)). ii) Non-treated epileptic rats. iii) Vehicle epileptic rats treated with 10% Polysorbate 20 (Tween 20). iv) TLE-treated rats with CIN once daily (50 mg/kg), three days after the first seizure and up to 28 days, four days a week (treatment group).<br> For the analysis, based on the Racine scale, the score of 4 and 5 was chosen. Rats were sacrificed and primed 28 days after the first seizure for both histopathological and quantitative real time PCR (qRT-PCR) analysis.<br> <strong><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span>&nbsp;</strong>The findings showed that CIN prevents cell death caused by Pilocarpine, via regulatory effect on apoptotic and anti-apoptotic gene expression. QRT-PCR results showed a significant increment in the <em>Bcl-2</em> expression, and a decrease in <em>Caspas-3</em> gene in the epileptic group treated with CIN. Also, amount of total antioxidant capacity was higher in CIN treated group. Histological study of the brain regions revealed a significant decrease in the apoptotic and necrotic hippocampal cells in the treatment groups.<br> <strong><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong>Collectively, the present study showed CIN significantly induced neuroprotection effects for brain damage. It seems CIN can be a promising method for improving the effectiveness of therapy.</span></span></p> 1, 8-cineole, Epilepsy, Pilocarpine, Bcl-2, Caspase-3 30 38 http://journal.zums.ac.ir/browse.php?a_code=A-10-41-5&slc_lang=en&sid=1 Mahsa Nikbakht Rad mahsanikbakht90@gmail.com 5200319475328460067440 5200319475328460067440 No Dept. of Anatomy, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Iraj Jafari Anarkooli anarkool@zums.ac.ir 5200319475328460067441 5200319475328460067441 Yes Dept. of Anatomy, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Alireza Abdanipour abdani.anatomy@yahoo.com 5200319475328460067442 5200319475328460067442 No Dept. of Anatomy, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran