en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong><span style="line-height:200%"><span style="line-height:200%"><span style="color:black">Despite the critical importance of catheter as an indwelling medical device, its prolonged utilization in hospitalized patients may lead to infection. This study aimed to identify distribution of uropathogenic bacteria isolated from catheterized uro-oncology patients, their biofilm production, and antimicrobial resistance patterns to generally -used antibiotics.</span></span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span>&nbsp;</strong><span style="line-height:200%"><span style="line-height:200%"><span style="color:black">The urine samples of catheterized urology cancer patients were collected for urinalysis and urine culture. Then capability of biofilm production was detected by Congo red agar method, tube method, and microtiter plate assay. Antimicrobial susceptibility test was also performed</span></span> <span style="line-height:200%"><span style="color:black">using the Kirby&ndash;Bauer disc diffusion method on Muller&ndash;Hinton agar. Subsequently, polymerase chain reaction (PCR) assays were used to detect the biofilm encoding genes.</span></span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span></strong>&nbsp;<span style="line-height:200%"><span style="line-height:200%"><span style="color:black">Of the 100 urinary catheter samples, 76 isolates were recovered from urinary catheters of 52 patients.</span></span> <i><span style="line-height:200%"><span style="color:black">Escherichia coli</span></span></i><span style="line-height:200%"><span style="color:black"> was established to be the most frequent pathogen isolated from the urine of patients followed by Pseudomonas and Staphylococcus. All of <i>Pseudomonas aeruginosa</i> and Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) isolates were found to be biofilm producers. All studied isolates were found resistant to ampicillin, amoxicillin, and cephalexin. All biofilm- producer MRSA and Pseudomonas isolates were found to harbor the virulence genes studied.</span></span> <span style="line-height:200%"><span style="color:black">Both imipenem and fosfomycin were the most effective antibiotics against isolated bacteria.</span></span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong><span style="line-height:200%"><span style="line-height:200%"><span style="color:black">In our study virulent pathogens with highly- resistant profile and potential to form biofilm were isolated from uro-oncology patients. Therefore, the current study highlights the significance of antibiotic resistance which can lead to treatment failure.</span></span></span></span></span></p> Prostate cancer, Urinary bladder cancer, Biofilm, Antimicrobial drug resistance 347 356 http://journal.zums.ac.ir/browse.php?a_code=A-10-5927-1&slc_lang=en&sid=1 Solmaz Ohadian Moghadam s-ohadian@sina.tums.ac.ir 5200319475328460073561 5200319475328460073561 Yes Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Nowroozi Mr.nowroozi@gmail.com 5200319475328460073562 5200319475328460073562 No Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran Ali Nowroozi nowrooziali77@gmail.com 5200319475328460073563 5200319475328460073563 No Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran Asieh Yousefi Kashi ausef1351@gmail.com 5200319475328460073564 5200319475328460073564 No Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran