en Pharmacology Pharmacology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong>Doxorubicin as an effective chemotherapeutic agent is frequently used in various cancers. Nowadays, the application of doxorubicin is limited due to its cardiotoxic effects. The important mechanism which is involved in the cardiac injury of&nbsp;doxorubicin is the generation of reactive oxygen species; therefore antioxidant compounds may reduce cardiotoxicity. In the present study, we evaluated the protective effects of <i>Portulaca oleracea</i> extract against doxorubicin-induced damage in cardiomyocytes cell line H9c2.<br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span>&nbsp;</strong><span style="letter-spacing:-.1pt">The H9c2 cells were pre-treated for 2h with different concentrations of extract (12-400&micro;g/ml) or resveratrol (50&micro;M, positive control), and then doxorubicin was added for 24h. Afterward, the cell viability, and parameters of oxidative stress including lipid peroxidation and reactive oxygen species (ROS) generation, and also apoptosis rate, were measured.</span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span></strong>&nbsp;<span style="letter-spacing:-.1pt">The results revealed that doxorubicin extremely decreased cell viability via increasing malondialdehyde, ROS, and apoptotic cells. The extract could reverse doxorubicin-induced cardiotoxicity through anti-oxidant activity.</span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong>In conclusion, we witnessed that <i>P. oleracea</i> has protective effect against doxorubicin-caused cardiomyocytes damage.</span></span></p> Cardioprotective agents, Doxorubicin, Cardiomyocytes , Portulaca oleracea 177 183 http://journal.zums.ac.ir/browse.php?a_code=A-10-6125-1&slc_lang=en&sid=1 Azar Hosseini hoseiniaz@mums.ac.ir 5200319475328460075597 5200319475328460075597 No Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran Mohaddeseh Sadat Alavi alavim942@mums.ac.ir 5200319475328460075598 5200319475328460075598 No Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran Soroush Mohammadi mohammadsorioush@gmail.com 5200319475328460075599 5200319475328460075599 No Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran Zahra Tayarani Najjaran tayaraninz@mums.ac.ir 5200319475328460075600 5200319475328460075600 No Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Arezoo Rajabian Rajabianar@mums.ac.ir 5200319475328460075601 5200319475328460075601 Yes Dept. of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran