en Pharmacology Pharmacology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong></span></span><span style="line-height:2;"><span style="font-size:16px;"><span style="font-family:Times New Roman;">Aging is a natural phenomenon which <span class="fontstyle01"><span style="font-weight:normal"><span style="font-style:normal">can cause changes in most </span></span></span>organs and cells. Numerous mechanisms including oxidative stress and free radical generation is involved in the progression of the aging process. Pomegranate seed oil (PSO), has different therapeutic properties including anti-oxidant and anti-inflammatory effects. In this research, the effect of PSO against D-galactose-induced aging is investigated.</span></span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span>&nbsp;</strong></span></span><span style="line-height:2;"><span style="font-size:16px;"><span style="font-family:Times New Roman;">D-galactose, 500 mg/kg, injected subcutaneously (S.C.) to induce aging in rats. Animals in treatment groups received PSO, 0.4 and 0.8 ml/kg intraperitoneally (i.p.). After 42 days, behavioral test was evaluated by passive avoidance (PA). Then animals killed, blood samples collected by cardiac puncture, and brain and liver removed. Levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) measured in serum. Malondialdehyde (MDA) and thiol contents of brain and liver homogenized tissue samples were determined.</span></span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span></strong>&nbsp;</span></span><span style="line-height:2;"><span style="font-size:16px;"><span style="font-family:Times New Roman;">D-galactose increased lipid-peroxidation in liver and brain tissues as well as elevation of ALT, AST, but the level of thiol contents decreased in homogenized tissues. Both doses of PSO attenuated d-galactose-induced injury in liver and brain by decreasing ALT, AST, MDA and elevation of thiol content. The PA test showed that PSO increased <span class="fontstyle01"><span style="font-weight:normal"><span style="font-style:normal">the latency time to enter the</span></span></span><br> <span class="fontstyle01"><span style="font-weight:normal"><span style="font-style:normal">dark chamber compared to the control group.</span></span></span></span></span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong></span></span><span style="line-height:2;"><span style="font-size:16px;"><span style="font-family:Times New Roman;">PSO decreased D-galactose-induced aging in rats via prevention of oxidative stress. This effect may be related to the presence of various compounds and their anti-oxidant properties, which is found in PSO.</span></span></span></p> Aging, Lipid peroxidation, Oxidative stress, Punica granatum 80 85 http://journal.zums.ac.ir/browse.php?a_code=A-10-6142-1&slc_lang=en&sid=1 Azar Hosseini hoseiniaz@mums.ac.ir 5200319475328460074796 5200319475328460074796 No Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran Leila Ziaee ziaeel2938@gmail.com 5200319475328460074797 5200319475328460074797 No Dept. of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Arezoo Rajabian rajabianar@mums.ac.ir 5200319475328460074798 5200319475328460074798 No Dept. of Internal Medicine, Faculty of Medicine, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran Mohammad Taher Boroushaki boroushakimt@mums.ac.ir 5200319475328460074799 5200319475328460074799 Yes Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran