en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong></span></span><span style="line-height:2;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="page:WordSection1">Dysregulation of long-term expression of non-coding RNAs (lncRNAs) has a potential role in progressive brain disorders such as Alzheimer&#39;s disease. This study aimed to analyze the apoptosis and expression of <i>51A</i> and <i>NAT-Rad18 </i>lncRNAs and their target genes in brain tissue and peripheral blood mononuclear cells (PBMCs) of the rat model of AD, before and after memantine treatment.</span></span></span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span>&nbsp;</strong></span></span><span style="line-height:2;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="letter-spacing:-.1pt">Twenty-eight male <i>Wistar</i> rats were divided into four groups: 1. Normal control (n = 4), 2. Sham-operated (n = 4), 3. Alzheimer&#39;s control (n = 10), and 4. The experimental group (n = 10) was treated with memantine. The qPCR and TUNEL tests were used to detect the lncRNAs expression and apoptosis.</span></span></span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span></strong>&nbsp;</span></span><span style="line-height:2;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="page:WordSection1"><i>Sorl1 </i>gene was reduced in brain tissue of Alzheimer&rsquo;s control (p = 0.016) and PBMCs of Alzheimer&#39;s control and experimental groups (p = 0.002 and p = 0.001 respectively). The expression of <i>NAT-Rad18</i> and <i>Rad18 </i>genes increased in brain tissue of Alzheimer&#39;s control group (p = 0.002 and p = 0.04 respectively) while reduced in PBMCs of Alzheimer&#39;s control and experimental groups (p = 0.005 and p = 0.045 for <i>NAT-Rad18</i>, p = 0.01 and p = 0.006 for <i>Rad18</i>).</span></span></span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong></span></span><span style="line-height:2;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="page:WordSection1">ROC curve analysis showed 100% sensitivity and 85.7% specificity for the <i>Sorl1</i> gene with 0.911 under the curve area and 100% sensitivity and 100% specificity for <i>NAT-Rad18</i> and <i>Rad18</i>, separately with one under the curve area. Decreased expression in <i>Sorl1</i>, <i>NAT-Rad18,</i> and <i>Rad18 </i>genes can be used as blood biomarkers for diagnosis independently. However, studies on Alzheimer&#39;s patients are needed.</span></span></span></span></p> Alzheimer’s disease, Rat, Biomarker, lncRNA, Memantine 86 92 http://journal.zums.ac.ir/browse.php?a_code=A-10-6202-1&slc_lang=en&sid=1 Parisa Azadfar Azadfar.parisa@gmail.com 5200319475328460074800 5200319475328460074800 No Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Zahra Noormohammadi z-nouri@srbiau.ac.ir 5200319475328460074801 5200319475328460074801 Yes Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Maryam Noroozian Maryam.Noroozian.mn@gmail.com 5200319475328460074802 5200319475328460074802 No Dept.of Memory and Behavioral Neurology (MBND), Roozbeh Hospital, Tehran, Iran Akram Eidi akram_eidi@yahoo.com 5200319475328460074803 5200319475328460074803 No Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Pejman Mortazavi sp.mortazavi@gmail.com 5200319475328460074804 5200319475328460074804 No Dept. of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran