en Life Science Life Science مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong>Infertility is a health problem that affects 72.4 million couples worldwide. Infertility can occur as a result of an unhealthy lifestyle, such as a smoking habit. Cigarettes contain nicotine, a toxic compound that can trigger oxidative stress and eventually decrease fertility. Temulawak is a medicinal plant that is rich in antioxidants and anti-inflammatory properties. It has been used as a traditional remedy and is believed to improve fertility. We aim to study the effect of temulawak extract on the morphology, motility, and sperm counts of nicotine-induced mice.<br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span>&nbsp;</strong><span style="letter-spacing:-.1pt">A total of 20 mice were divided into four groups (n = 5): control normal (K0); nicotine-induced (K1); and nicotine-induced treated with two different concentrations of temulawak extract, 4 mg/20 g body weight (P1) and 8 mg/20 g body weight (P2). The mice were given nicotine and temulawak for 28 days and then killed by neck dislocation. Both testes and vas deferens were collected for sperm analysis.</span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span></strong>&nbsp;<span style="letter-spacing:-.1pt">Mice treated with<b> </b>nicotine (K1) showed lower sperm counts <i>(p=0.012) </i>and motility <i>(p<0.001</i>) compared to mice in the control normal (K0) but had no difference in morphology. Treatment with temulawak (4 mg/20 g body weight) on nicotine-induced mice (P1) significantly increased sperm motility <i>(p< 0.001).</i> A double dose of temulawak extract (P2) resulted in significantly higher motility and sperm counts than in group K1.</span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong>Temulawak treatment (8 mg/20 g body weight) on nicotine-induced mice can significantly improve motility and sperm counts.</span></span></p> Nicotine, Temulawak, Spermatozoa, Infertility 191 196 http://journal.zums.ac.ir/browse.php?a_code=A-10-6271-1&slc_lang=en&sid=1 Dimas Sindhu Wibisono uroandrology21@gmail.com 5200319475328460075606 5200319475328460075606 Yes Dept. of Urology, Kariadi Hospital, Faculty of Medicine, Diponegoro University, Semarang, Indonesia Josephine Fiona Sucahyo josephinefiona24@gmail.com 5200319475328460075607 5200319475328460075607 No Faculty of Medicine, Diponegoro University, Semarang, Indonesia Faizah Fulyani f.fulyani@fk.undip.ac.id 5200319475328460075608 5200319475328460075608 No Dept. of Medical Biology and Biochemistry, Faculty of Medicine, Diponegoro University, Semarang, Indonesia Mahayu Dewi Ariani mahayudewi@gmail.com 5200319475328460075609 5200319475328460075609 No Dept. of Medical Biology and Biochemistry, Faculty of Medicine, Diponegoro University, Semarang, Indonesia Eriawan Agung Nugroho eriawanspu@gmail.com 5200319475328460075610 5200319475328460075610 No Dept. of Urology, Kariadi Hospital, Faculty of Medicine, Diponegoro University, Semarang, Indonesia Nanda Daniswara uronanda@gmail.com 5200319475328460075611 5200319475328460075611 No Dept. of Urology, Kariadi Hospital, Faculty of Medicine, Diponegoro University, Semarang, Indonesia