en Clinical Medicine Clinical Medicine مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong><span style="line-height:200%"><span style="unicode-bidi:embed">Trace element levels are important for sperm function. On the other hand, oxidative stress is one of the most important causes of DNA damage. This study aimed to evaluate the relationship between cadmium (Cd), nickel (Ni), and manganese (Mn) trace elements and also oxidative stress in infertile men.</span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span>&nbsp;</strong><span style="line-height:200%"><span style="unicode-bidi:embed">This case-control study was carried out on 50 oligozoospermic, 50 asthenozoospermic, and 50 normozoospermic men. All individuals were subjected to semen analysis. Cadmium, nickel, manganese, total antioxidant capacity (TAC), and total oxidant status (TOS) were detected using the manual assays. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were analyzed using the ELISA.</span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span></strong>&nbsp;<span style="line-height:200%"><span style="unicode-bidi:embed">The findings revealed that there was a significant increase in TOS index in the patient groups compared to the normozoospermic group (p = 0.045, p = 0.038 respectively); however, these changes were downward for the TAC factor. The levels of SOD and GPx activities were significantly reduced in the patient groups compared to the normozoospermic group (p = 0.015, p = 0.020 respectively). Also, Cd and Ni levels were significantly elevated and had a negative association with TAC in the patient groups compared to the normozoospermic group. However, the results of Mn level showed a significantly lower value and a positive association with the TAC index.</span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong><span style="line-height:200%"><span style="unicode-bidi:embed">Mn as a component of SOD enzyme is necessary for normal sperm functions. In contrast, high levels of Cd and Ni are toxic for human sperm and negatively correlated with TAC and sperm parameters.</span></span><span style="line-height:200%"><span style="unicode-bidi:embed"> </span></span></span></span></p> Trace Elements, Oxidative Stress, Oligozoospermia, Asthenozoospermia. Normozoospermia 372 380 http://journal.zums.ac.ir/browse.php?a_code=A-10-6339-1&slc_lang=en&sid=1 Alireza Gharabaghi ar.gharebaghi@gmail.com 5200319475328460077757 5200319475328460077757 No School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran Mohammad Sajjad Yavari Bazl yavarybazl@gmail.com 5200319475328460077758 5200319475328460077758 No School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran Khadijeh Baranizadeh baranyzadeh@gmail.com 5200319475328460077759 5200319475328460077759 No Dept. of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran Ali Heshmati ali_heshmaati@yahoo.com 5200319475328460077760 5200319475328460077760 No Dept. of Nutritional Sciences, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran Gholamreza Shafiee g.r_shafiee@yahoo.com 5200319475328460077761 5200319475328460077761 Yes Dept. of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran