en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong></span></span><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:150%"><span style="font-size:16px;"><span style="line-height:150%">Acute myeloid leukemia (AML) has a complex course of treatment, including chemotherapy and bone marrow transplantation, which mostly results in drug resistance and recurrence. Therefore, alternative therapies have attracted the attention of researchers. Mesenchymal stem cells exert their paracrine effects through the secretion of multiple cytokines and vesicles. Recent findings suggest the possible antitumor properties of MSCs by the secretion of</span> <span style="line-height:150%">micro-vesicles, and consequent activation of cell death pathways in cancer cells. </span>The present study evaluated how micro-vesicles from human placental mesenchymal stem cells affect the autophagy and apoptosis pathways in AML.</span></span></span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span>&nbsp;</strong></span></span><span style="font-family:Times New Roman;"><span style="font-size:16px;"><span style="line-height:150%"><span style="line-height:150%">After isolation and culture, hPMSCs were identified through flow cytometry. The Bradford method was employed to determine the concentration of MVs. The properties of MVs were confirmed by transmission electron microscopy (TEM) and DLS.</span> Next, the KG1 cell line was exposed to MVs at concentrations of 25, 50, and 100 &mu;g/ml for 24 hours. Subsequently, apoptosis was assessed using an Annexin V-FITC/PI kit, and ROS activity was gauged through the utilization of H2DCFDA. The gene expression in the autophagy and apoptosis pathways was investigated by using the real-time PCR technique.</span></span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span></strong>&nbsp;</span></span><span style="font-family:Times New Roman;"><span style="font-size:16px;"><span style="line-height:150%"><span style="line-height:150%">After 24 hours of treatment, </span>a rise <span style="line-height:150%">in intracellular ROS accumulation and apoptosis was observed</span> in all groups compared to the control group<span style="line-height:150%">. The mean intracellular ROS accumulation at the concentrations of 25, 50, and 100 &mu;g / ml</span> <span style="line-height:150%">of the samples and the control group was 62.21% (<i>P</i><0.0002), 66.25% (<i>P</i><0.0001), 62.55% (<i>P</i><0.0001), and 26.1% (<i>P</i><0.0001), respectively. The apoptosis indices at the concentrations of 25, 50, and 100 &mu;g / ml of the samples were 62.6% (<i>P</i><0.0002), 46.0% (<i>P</i><0.0001), and 48.2% (<i>P</i><0.0001), respectively. </span>Furthermore, analyzing genes through RT-PCR indicated a considerable increase in the expression of autophagy-related and pro-apoptotic genes.</span></span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong></span></span><span style="font-family:Times New Roman;"><span style="font-size:16px;"><span style="line-height:150%"><span style="line-height:150%">Our findings indicate that hPMSC-MVs induce Cell death pathways of autophagy and apoptosis in the KG1 cell lines.</span> Additionally, hPMSC-MVs exhibited heightened impactful anti-proliferative and pro-apoptotic outcomes on KG1 cells in vitro<span style="line-height:150%">.</span></span></span></span></p> <p style="text-align: justify;"></p> Placental Mesenchymal Stem Cell, Micro-vesicles, Acute Myeloid Leukemia, Autophagy, Apoptosis 574 584 http://journal.zums.ac.ir/browse.php?a_code=A-10-6557-1&slc_lang=en&sid=1 Jamal Motallebzadeh Khanmiri jamkhanmiri@gmail.com 5200319475328460079154 5200319475328460079154 No Dept. of Hematology and Blood Transfusion Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran Seyed Hadi Mousavi hmousavi@tums.ac.ir 5200319475328460079155 5200319475328460079155 Yes Dept. of Hematology and Blood Transfusion Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran Shaban Alizadeh alizadehs@tums.ac.ir 5200319475328460079156 5200319475328460079156 No Dept. of Hematology and Blood Transfusion Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran Mohammad Khani-Eshratabadi Lskhani@yahoo.com 5200319475328460079157 5200319475328460079157 No Dept. of Hematology and Blood Transfusion Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran