en Pharmacology Pharmacology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong></span></span><span style="font-family:Times New Roman;"><span style="font-size:16px;"><span style="line-height:180%"><i>Peganum harmala</i> has emerged as a promising anti-diabetic medicine. There is no study regarding the impact of <i>P. harmala</i> concentration on the insulin secretion, C-peptide secretion, and glucose uptake. We investigated the effect of different concentrations of methanolic extracts of <i>P. harmala </i>seed and leaf on insulin and C-peptide secretion, and glucose uptake.</span></span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span>&nbsp;</strong></span></span><span style="font-family:Times New Roman;"><span style="font-size:16px;"><span style="line-height:180%">After the cell passaging, pancreatic carcinoma cell line (PANC-1) and HT1080 were treated with different concentrations of seed and leaf extract of <i>P. harmala</i>, harmine, and ghrelin agonists. The <span style="background:white">MTT</span> was employed to assess the cell survival at the selective doses, and using a spectrophotometer, the absorbance was determined at 570 nm. After 72-h treatment, the insulin and C-peptide secretion were measured by ELISA. To measure the intracellular glucose concentrations after treating muscle carcinoma cell lines, glucose oxidase method was utilized.</span></span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span></strong>&nbsp;</span></span><span style="font-family:Times New Roman;"><span style="font-size:16px;"><span style="line-height:180%"><i>P. harmala </i>seed and leaf extracts increased the secretion of insulin and C-peptide in a dose-dependent manner compared to ghrelin and harmine. These extracts increased the intracellular glucose concentration at high doses (150 and 1500 &micro;g/ml for the seed and leaf extract, respectively) of HT1080 cell line. However, their high concentration was toxic and reduced the cell survival. The methanolic extracts of seed showed a higher insulin (17.5-fold) and C-peptide (7.8-fold) secretion compared to the leaf methanolic extracts.</span></span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong></span></span><span style="font-family:Times New Roman;"><span style="font-size:16px;"><span style="line-height:180%">Due to the presence of &beta;-carbolines, the <i>P. harmala </i>seed extract has toxicity and affects insulin secretion and C-peptide uptake secretion at lower concentrations than those of leaf extract.</span></span></span></p> <p style="text-align: justify;"></p> Carcinoma, C-peptide, Peganum, Insulin 585 593 http://journal.zums.ac.ir/browse.php?a_code=A-10-6658-1&slc_lang=en&sid=1 Alieh Safamanesh safamaneshalieh@gmail.com 5200319475328460079142 5200319475328460079142 Yes Dept. of Animal Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran Shahrbanoo Oryan sh_oryan@khu.ac.ir 5200319475328460079143 5200319475328460079143 No Dept. of Animal Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran Ramesh Ahmadi ramahmd@yahoo.com 5200319475328460079144 5200319475328460079144 No Dept. of Animal Sciences, Faculty of Basic Sciences, Islamic Azad University of Qom, Qom, Iran Kazem Parivar k.parivar@srbiau.ac.ir 5200319475328460079145 5200319475328460079145 No Dept. of Animal Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran