en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Background and Objective:</span></span></span></b> <span new="" roman="" style="font-family:" times="">Investigating multidrug resistance and TEM and SHV broad-spectrum beta-lactamase genes in Escherichia coli bacteria isolated from patients with urinary tract infections is very useful to improve the treatment of infection and prevent the failure of treatment of urinary tract infections.</span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">The aim of this study was to investigate multidrug resistance and TEM and SHV broad-spectrum beta-lactamase genes in <i>Escherichia coli</i> bacteria isolated from patients with urinary tract infections. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Materials and Methods: </span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">In this study, 188 strains of <i>Escherichia coli</i>, which cause urinary tract infections in Alborz province, were studied. Urine samples were cultured on EMB and Blood Agar media. Differential tests were performed for final identification. ESBL-producing strains were identified, PCR was performed to survey the abundance of ESBL-producing genes.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Based on the results of the disk diffusion and Double-disk synergy testS, 82 (43.6%) strains were determined as the final producer of ESBL. out of these isolates, the frequency of SHV, TEM, and CTX genes measured 64.3%, 55.9%, and 21.4%, respectively. These results showed that 12 (14.28%) of <i>Escherichia coli</i> isolated have all genes, 26 (30.95%) had 2 genes and 36 (42.85%) had one gene.</span></span></span><span style="font-size:14.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">According to the results, it was found that imipenem with the lowest resistance is the best drug in the treatment, and carbapenems are the best drug for treating diseases caused by <i>Escherichia coli</i>. The results of the current study may be useful in replacing ESBL enzyme resistance screening with more modern sensitivity measurement methods such as MIC and Etest.</span></span></span></span></span></span> Escherichia coli, Drug resistance, Beta-lactamases, Extended-spectrum beta-lactamases (ESBLs) 79 86 http://journal.zums.ac.ir/browse.php?a_code=A-10-460-5&slc_lang=en&sid=1 Mohammad Mehdi Soltan- Dallal msoltandallal@gmail.com 5200319475328460080477 5200319475328460080477 Yes Food Microbiology Research Center, Tehran University of Medical Sciences, Iran Seyedeh Zohre Mirbagheri z_mirbagheri@hotmail.com 5200319475328460080478 5200319475328460080478 No Department of Pathobiology, School of Health, Tehran University of Medical Sciences, Tehran, Iran Saeed Vahedi vahedisaeid@yahoo.com 5200319475328460080479 5200319475328460080479 No Instructor, Department of Anesthesiology, School of Paramedical Sciences, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Mohammadi mreza_mohammadi@modares.ac.ir 5200319475328460080480 5200319475328460080480 No Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Hedroosha Molla Agha Mirzaei hedroosh.mirzaei@gmail.com 5200319475328460080481 5200319475328460080481 No Master of Microbiology, Food Microbiology Research Center, Tehran University of Medical Sciences,Tehran, Iran.