en Life Science Life Science مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="line-height:150%"><b><span style="line-height:150%">ackground and Objective:</span></b><span style="line-height:150%"> Bisphenol A </span><span style="background:white"><span style="color:black">[BPA; 2,2-bis-(4 hydroxyphenyl) propane]</span></span><span style="line-height:150%"> is an environmental estrogenic and endocrine-disrupting compound that exerts its destructive effects through increasing oxidative stress, but the mechanisms underlying this effect have not yet been fully explained. This study evaluates BPA toxicity in muscle cells and the therapeutic potential of adiponectin (APN) and quercetin (QUER).</span></span><br> <span style="line-height:150%"><b><span style="line-height:150%">Materials & Methods:</span></b><span style="line-height:150%"> Confluent L6 rat muscle cells were exposed to BPA (50 and 100 &mu;M) with APN (10 and 100 ng/ml) and QUER (10 and 25 </span><span style="line-height:150%">mM) for 24 and 48 hours. Cell viability, pro-oxidant/antioxidant balance (PAB), catalase (CAT) activity, and KEAP1/Nrf2 gene expression were analyzed using desired methods. </span></span><br> <span style="line-height:150%"><b><span style="line-height:150%">Results</span></b><span style="line-height:150%">: BPA in high doses (100 </span><span style="line-height:150%">m</span><span style="line-height:150%">M) significantly reduced the viability of the muscle cells, while APN and QUER increased cell survival in a time-dependent manner. The effect of QUER also was dose-dependent</span><span style="line-height:150%">, while APN in high doses decreased the viability of the muscle cells. APN and QUER also reduced BPA-associated oxidative stress. These changes were significant in the case of QUER. The CAT activity was reduced in BPA-treated cells, which was notably increased with APN and QUER treatment. A decrease in Nrf2 gene expression of BPA-treated muscle cells improved by treatment with QUER and APN in a dose-dependent manner.</span></span><br> <span style="line-height:150%"><b><span style="line-height:150%">Conclusion:</span></b><span style="line-height:150%"> Our results hinted that APN and QUER could modulate BPA-induced oxidative stress in muscle cells through KEAP1/Nrf2 pathways. </span><span style="line-height:150%">Accordingly, it can also be concluded that APN in low doses and QUER may significantly reduce muscle toxicity caused by BPA.</span></span></span></span></p> Adiponectin, Flavonoid, Oxidative stress, Muscle cell toxicity, Bisphenol A 48 59 http://journal.zums.ac.ir/browse.php?a_code=A-10-5711-2&slc_lang=en&sid=1 Davoud Mohammadi davoud.mohammadi89@gmail.com 5200319475328460089214 5200319475328460089214 No Department of Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Farideh Jalali-Mashayekhi mashayekhif@yahoo.com 5200319475328460089215 5200319475328460089215 No Department of Biochemistry and Genetics, School of Medicine, Arak University of Medical Sciences, Arak, Iran Keivan Nedaei nedaee.k@gmail.com 5200319475328460089216 5200319475328460089216 No Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Mina Hemmati mina1hemmati@yahoo.com 5200319475328460089217 5200319475328460089217 Yes Department of Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Darya Ghadimi darya.ghadimi@gmail.com 5200319475328460089218 5200319475328460089218 No Department of Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Zahra Rasooli zahrarasooli68@gmail.com 5200319475328460089219 5200319475328460089219 No Department of Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran