en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="line-height:150%"><b><span new="" roman="" times=""><span style="color:#0e101a">Background:</span></span></b><span new="" roman="" times=""><span style="color:#0e101a"> Breast cancer (BrCa) is the most prevalent tumor among women globally, aside from nonmelanoma skin malignancies. Unfortunately, many BrCa patients exhibit a poor response to immune checkpoint inhibitors (ICIs) because of a deficiency of infiltrating immune cells. Earlier research has shown that guanylate-binding protein 1 (GBP1) can modify the inhibitory properties of inflammatory cytokines on endothelial cell proliferation, immigration, and invasion.</span></span></span><br> <span style="line-height:150%"><b><span new="" roman="" times=""><span style="color:#0e101a">Objective:</span></span></b> <span new="" roman="" times="">Since the fundamental functions of the GBP family in the cancer-immune cycle is uncertain, according to recent findings, the function of GBPs in BrCa was studied. However, the underlying function of the GBP family in the cancer-immunity cycle is uncertain. We decided to study GBPs&rsquo; role in BrCa.<span style="color:#0e101a"></span></span></span><br> <span style="line-height:150%"><b><span new="" roman="" times=""><span style="color:#0e101a">Methods:</span></span></b><span new="" roman="" times=""><span style="color:#0e101a"> We applied GEPIA, UALCAN, cBioPortal, GeneMANIA, Kaplan-Meier plotter, Human Protein Atlas, STRING, and TIMER in our analysis.</span></span></span><br> <span style="line-height:150%"><b><span new="" roman="" times=""><span style="color:#0e101a">Results:</span></span></b><span new="" roman="" times=""><span style="color:#0e101a"> Our results confirmed a strong relation between the expression of GBP1/GBP2/GBP3/GBP4/GBP5/GBP6/GBP7 and the infiltration of all immune cells. Specifically, high GBP1/4/5 expression was strongly linked with increased infiltration of dendritic cells, B cells, CD4+ T cells, CD8+ T cells, and neutrophils in BrCa, whereas GBP2/3/6/7 expression showed a weaker positive correlation.</span></span></span><br> <span style="line-height:150%"><b><span new="" roman="" times=""><span style="color:#0e101a">Conclusion:</span></span></b><span new="" roman="" times=""><span style="color:#0e101a"> These results suggest a therapeutic assessment for the GBP family in combination with ICIs for treating BrCa.</span></span></span></span></span></p> Prognostic, Guanylate-Binding Proteins, Immune Infiltrates, Breast Cancer 18 27 http://journal.zums.ac.ir/browse.php?a_code=A-10-5411-3&slc_lang=en&sid=1 Samaneh Biglari samaneh.biglari92@gmail.com 5200319475328460082805 5200319475328460082805 No Department of Genetics and Molecular Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Haleh Siami haleh.siami@yahoo.com 5200319475328460082806 5200319475328460082806 No School of Medicine, Islamic Azad university of Medical Science, Tehran, Iran Zahra Ghahremani ghahramaniz@zums.ac.ir 5200319475328460082807 5200319475328460082807 No Department of Radiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Negin Parsamanesh neginparsa.684@gmail.com 5200319475328460082808 5200319475328460082808 Yes Department of Genetics and Molecular Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran