en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="background:#2d7f8f"><span style="color:white">Background & Objective:</span></span></span></b><b> </b><b>&nbsp;</b><span style="font-size:9.0pt"><span style="color:black">Colorectal cancer (CRC) is one of the most common types of cancer in the world and is considered a leading cause of cancer-related death. The present study aimed to investigate the inhibitory effect of<i> lactobacillus acidophilus</i> (PTCC 1643) supernatant (LAS) and <i>lactobacillus rhamnosus</i> (PTCC 1657) supernatant (LRS) on the growth and invasiveness of the human colon carcinoma cell line (Caco2) in-vitro. </span></span></span></span><br> <span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="background:#2d7f8f"><span style="color:white"><span style="letter-spacing:-.1pt">&nbsp;Materials & Methods:</span></span></span></span></b> &nbsp;<span style="font-size:9.0pt"><span style="color:black"><span style="letter-spacing:-.1pt">In this study, the antiproliferative activity and anti-invasion potential of LAS and LRS were determined by MTT and transwell chamber assays, respectively. Furthermore, the expression of mitochondrial membrane potential-9 (MMP-9) and matrix metalloproteinase-12 (MMP12) genes were analyzed by real-time PCR.</span></span></span></span></span><br> <span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span lang="EN-IN" style="font-size:9.0pt"><span style="background:#2d7f8f"><span style="color:white">Results: </span></span></span></b><b>&nbsp;</b><span style="font-size:9.0pt"><span style="color:black"><span style="letter-spacing:-.1pt">The results of the MTT assay indicated that LAS and LRS had cytotoxic effects on Caco-2 cell proliferation at a concentration of 25% and higher after 72 hours (<i>p</i><0.0001). Thus, the minimum concentrations (25%) of supernatants were chosen for further experiments. LRS and LAS could significantly suppress the invasiveness of cells, <i>p</i>= 0.028 and <i>p</i>=0.002, respectively. Also, the expression of MMP12 was significantly increased in cells when treated with LAS (<i>p</i><0.001), whereas LRS had no significant effect on MMP-12 expression. Furthermore, the expression of MMP-9 was statistically decreased in cells treated with both supernatants (<i>p</i><0.00001).</span></span></span></span></span><br> <span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="background:#2d7f8f"><span style="color:white">Conclusion: </span></span></span></b><b>&nbsp;</b><span style="font-size:9.0pt"><span style="color:black">In general, it was shown that LAS and LRS exert anti-cancer activity against the growth, invasion, and metastasis of Caco2 cells. </span></span></span></span><br> &nbsp; Lactobacillus, Colon cancer, Probiotic, Invasion assay, Metastasis 449 457 http://journal.zums.ac.ir/browse.php?a_code=A-10-6921-1&slc_lang=en&sid=1 Ameneh Shokati amenesh113@gmail.com 5200319475328460089675 5200319475328460089675 No Department of Hematology and Cell Therapy, Faculty of Medical Science, Tarbiat Modares University, Tehran, 14117-13116, Iran Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Masoud Soleimani soleim_m@modares.ac.ir 5200319475328460089676 5200319475328460089676 No Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Saeid Abroun abroun@modares.ac.ir 5200319475328460089677 5200319475328460089677 Yes Department of Hematology and Cell Therapy, Faculty of Medical Science, Tarbiat Modares University, Tehran, 14117-13116, Iran.