en Life Science Life Science مقاله پژوهشی Original Research Article <span style="font-size:11pt"><span style="line-height:200%"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Background</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">: Short tandem repeats (STR) are highly polymorphic genetic markers widely used in human identification. This study aimed to investigate the genetic characteristics of 21 autosomal STR loci in individuals from eastern Iran</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">.</span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Methods</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">: Sixty healthy volunteers from twenty families provided buccal samples for analysis using the PowerPlex&reg; 21 System. Various genetic and forensic parameters, including polymorphic information content, random matching probability, allelic discrimination power, paternity index, and exclusion power, were assessed</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">.</span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Results</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">: Five STR markers (D1S1656, D6S1043, D12S391, Penta D, and Penta E) were identified as prevalent in the eastern Iranian population, enabling successful parentage verification. All loci were in Hardy-Weinberg equilibrium, with a total of 178 alleles detected. The Penta E locus had the highest number of alleles, while TPOX had the most frequent allele. D12S391 exhibited the highest heterozygosity percentage (96.7%) among the loci analyzed</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">.</span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Conclusion</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">: The study confirmed the high informativeness of the 20 autosomal STR markers in individuals from eastern Iran, supporting previous findings. These results contribute to the genetic characterization of the eastern Iranian population and highlight the utility of these markers in forensic applications.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br> &nbsp; Forensic investigation, Short Tandem Repeat, Eastern Iran, Population genetics, Genetic markers 370 377 http://journal.zums.ac.ir/browse.php?a_code=A-10-7014-1&slc_lang=en&sid=1 Saeed Nasseri s.nasseri@bums.ac.ir 5200319475328460089563 5200319475328460089563 No Department of Molecular Medicine, Cellular & Molecular Research Center, School of Medicine, Birjand University of Medical Sciences, South Khorasan, Birjand, Iran Farzane Vafaeie farzanevafaeie@gmail.com 5200319475328460089564 5200319475328460089564 No Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran Ebrahim Miri-Moghaddam miri4@bums.ac.ir 5200319475328460089565 5200319475328460089565 Yes Department of Molecular Medicine, Faculty of Medicine, and Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran