en Pharmacology Pharmacology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#2d7f8f"><span style="color:white">Background & Objective:</span></span><b> </b>&nbsp;</b><span style="color:black">Doxorubicin (Dox) is used in chemotherapy, but its usage is restricted due to cardiotoxicity. <i>Sanguisorba minor</i> (<i>S. minor</i>) and chlorogenic acid (CGA) exhibit pharmacological activities, including the reduction of oxidative stress and induction of programmed cell death. In this investigation, the cardioprotective effects of <i>S. minor</i> and CGA were evaluated following Dox-stimulated toxicity in rats.</span><br> <b><span style="background:#2d7f8f"><span style="color:white"><span style="letter-spacing:-.1pt">&nbsp;Materials & Methods:</span></span></span></b> &nbsp;<span style="color:black"><span style="letter-spacing:-.1pt">Forty male Wistar rats were used in this research. Saline was administered to the control group; Dox (2.5 mg/kg, intraperitoneally, on alternate days) was injected to the toxicity group; In the second week, groups III and IV received oral <i>S. minor</i> extract at 100 or 300 mg/kg; and Group V received CGA (40 mg/kg, intraperitoneally). Serum levels of lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) were quantified as cardiac injury markers. Cardiac tissues were analyzed for superoxide dismutase (SOD) activity, malondialdehyde (MDA) levels, and thiol content. The modification of histopathology was studied via hematoxylin and eosin (H&E) staining.</span></span><br> <b><span lang="EN-IN"><span style="background:#2d7f8f"><span style="color:white">Results: </span></span></span>&nbsp;</b><span style="color:black"><span style="letter-spacing:-.1pt">Dox increased LDH, CK-MB, and MDA levels and reduced SOD and thiols. The administration<i> </i>of <i>S. minor</i> and CGA ameliorated Dox-induced biochemical changes. Histopathological analysis demonstrated extracellular edema, moderate congestion, and localized hemorrhage following Dox exposure. These effects were mitigated by treatment with <i>S. minor</i> and CGA.</span></span><br> <b><span style="background:#2d7f8f"><span style="color:white">Conclusion: </span></span>&nbsp;</b><span style="color:black">The administration of <i>S. minor</i> and CGA provided significant protection against Dox-related cardiotoxicity, likely through the modulation of oxidative stress pathways and preservation of myocardial structure.</span></span></span></p> Doxorubicin, Chlorogenic Acid, Sanguisorba minor, Cardiotoxicity, Oxidative Stress, Antioxidant Agent 156 166 http://journal.zums.ac.ir/browse.php?a_code=A-10-2535-2&slc_lang=en&sid=1 Mohaddeseh Sadat Alavi alavimhd@mums.ac.ir 5200319475328460087096 5200319475328460087096 No Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran Fereshte Torabi torabif991@mums.ac.ir 5200319475328460087097 0009-0007-2569-1118 No Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Pegah Yazdanpanah yazdanpanahp2@mums.ac.ir 5200319475328460087098 0009-0005-3825-246X No Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran Azar Hosseini hoseiniaz@mums.ac.ir 5200319475328460087099 5200319475328460087099 Yes Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran