en Pharmacology Pharmacology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#2d7f8f"><span style="color:white">Background & Objective:</span></span><b> </b></b>Cross-talk between endoplasmic reticulum (ER) stress and oxidative stress has been recognized as contributing to several pathological conditions, particularly those affecting the respiratory system. This study aims to investigate the potential protective role of chlorogenic acid (CA) against oxidative stress in a murine model of ER stress induced by tunicamycin.<br> <b><span style="background:#2d7f8f"><span style="color:white"><span style="letter-spacing:-.1pt">&nbsp;Materials & Methods:</span></span></span></b> <span style="letter-spacing:-.2pt">36 male C57BL/6</span><span style="letter-spacing:-.1pt"> mice were divided into six groups: a control group (saline), a vehicle group (DMSO), a CA group, a tunicamycin group, a CA + tunicamycin group, and a CA + tunicamycin group (both with a 20 mg/kg CA dose). After 72 hours of treatment, protein levels were investigated by ELISA, mRNA levels by RT-PCR, and histopathological alterations were examined by H&E staining.</span><span style="color:black"><span style="letter-spacing:-.1pt"> </span></span><br> <b><span lang="EN-IN"><span style="background:#2d7f8f"><span style="color:white">Results: </span></span></span>&nbsp;</b><span style="letter-spacing:-.1pt">We found that 20 mg/kg CA alleviated GRP78 mRNA levels, decreased MDA, and increased GSH levels. It also showed potent anti-inflammatory and anti-fibrotic effects in the lungs of ER-stress induced mice.</span><br> <b><span style="background:#2d7f8f"><span style="color:white">Conclusion: </span></span>&nbsp;</b>Chlorogenic acid administration decreased oxidative stress markers by alleviating ER stress. This indicates a mechanistic link between ER stress and oxidative stress.</span></span></p> Chlrogenic Acid, Endoplasmic Reticulum Stress, Oxidative Stress, Lung 157 163 http://journal.zums.ac.ir/browse.php?a_code=A-10-7190-1&slc_lang=en&sid=1 Azam Moslehi Moslehi2000@gmail.com 5200319475328460084328 5200319475328460084328 No Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran Tahereh Komeili Movahhed t_komeili_m@yahoo.com 5200319475328460084329 5200319475328460084329 No Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran Fatemeh Heidari heidari.anatomy@gmail.com 5200319475328460084330 5200319475328460084330 Yes Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran