en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#2d7f8f"><span style="color:white">Background & Objective:</span></span><b> </b>&nbsp;</b><span style="color:black">Atopy is an allergic state with manifestations of asthma, atopic dermatitis, and allergic rhinitis. There was a genetic tendency to atopy, and the genetic polymorphisms in the <i>TLR</i>s had been shown as a causal factor. The objective of this research was to examine the association between <i>TLR2</i> rs7656411 polymorphism and atopic individuals in Zanjan Province.</span><br> <b><span style="background:#2d7f8f"><span style="color:white"><span style="letter-spacing:-.1pt">&nbsp;Materials & Methods:</span></span></span></b> &nbsp;<span style="color:black"><span style="letter-spacing:-.1pt">Total genomic DNA was extracted from the whole blood of atopic and non-atopic individuals (100 atopic patients and 50 healthy control subjects) who were referred to the Allergy Clinic at Mousavi Hospital of Zanjan University of Medical Sciences Zanjan, Iran. PCR amplification and direct sequencing methods were employed for genotyping of <i>TLR2</i> rs7656411 in all patients and controls.</span><span style="letter-spacing:-.1pt"></span></span><br> <b><span lang="EN-IN"><span style="background:#2d7f8f"><span style="color:white">Results: </span></span></span>&nbsp;</b><span style="color:black"><span style="letter-spacing:-.1pt">In this study, the GG genotype was higher in the patients than in the controls (15% vs. 8% in non-atopic subjects). However, this difference was not significant (P-value = 0.21). We also did not find any significant difference between the frequency of alleles (G and T) in the patients and controls.</span></span><br> <b><span style="background:#2d7f8f"><span style="color:white">Conclusion: </span></span>&nbsp;</b><span style="color:black">Our study showed that <i>TLR2</i> rs7656411 polymorphism are not significantly associated with atopy in an Iranian population, but the GG genotype might be a risk factor for atopy.</span></span></span></p> Asthma, TLR2, Single Nucleotide Polymorphism, Atopy 291 299 http://journal.zums.ac.ir/browse.php?a_code=A-10-912-2&slc_lang=en&sid=1 Akefeh Ahmadiafshar akefeh45@zums.ac.ir 5200319475328460085807 5200319475328460085807 Yes Social Determinants of Health Research Center and Metabolic Diseases Research Center, Zanjan University of Medical Sciences, Zanjan, Iran Fatemeh Mohajer fateme.mohajer@gmail.com 5200319475328460085808 5200319475328460085808 No Mousavi Hospital, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Nima Motamed nima.motamed@zums.ac.ir 5200319475328460085809 5200319475328460085809 No Department of Community Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Roghayeh Arezumand arezumand1984@gmail.com 5200319475328460085810 5200319475328460085810 No Department of Medical Biotechnology and Molecular Science, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran Ali Ramazani Ramazania@zums.ac.ir 5200319475328460085811 5200319475328460085811 No Department of Pharmaceutical Biotechnology, Zanjan University of Medical Sciences, Zanjan, Iran