en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#2d7f8f"><span style="color:white">Background & Objective:</span></span><b> </b>&nbsp;</b><span style="color:black">Acute myeloid leukemia (AML) is a diverse blood cancer that predominantly affects adults. In addition to genetic mutations, epigenetic processes, including DNA methylation and histone modifications are key factors in leukemogenesis. This study investigated the relationship between the <i>Suppressor of Cytokine Signaling 1 (SOCS1)</i> gene methylation and the <i>Enhancer of Zeste Homolog 2 (EZH2)</i> gene expression in patients with AML.</span><br> <b><span style="background:#2d7f8f"><span style="color:white"><span style="letter-spacing:-.1pt">&nbsp;Materials & Methods:</span></span></span></b> &nbsp;<span style="color:black"><span style="letter-spacing:-.1pt">This cross-sectional study included 85 AML patients admitted to Ghaem Hospital (Mashhad, Iran) between April 2017 and March 2023. Patients were diagnosed based on the World Health Organization (WHO) guidelines and the French-American-British (FAB) classification. The <i>EZH2</i> expression level and <i>SOCS1</i> methylation patterns were analyzed. The associations between these epigenetic alterations, demographic characteristics, and clinical outcomes were assessed.</span></span><br> <b><span lang="EN-IN"><span style="background:#2d7f8f"><span style="color:white">Results: </span></span></span>&nbsp;</b><span style="color:black"><span style="letter-spacing:-.1pt">Our findings indicated a significant inverse relationship between <i>EZH2</i> expression and <i>SOCS1</i> gene methylation. Furthermore, <i>EZH2</i> overexpression and <i>SOCS1</i> hypomethylation were associated with reduced overall survival.</span></span><br> <b><span style="background:#2d7f8f"><span style="color:white">Conclusion: </span></span>&nbsp;</b><span style="color:black">This study suggests that <i>EZH2</i> and <i>SOCS1</i> may jointly influence AML progression and patient outcomes through epigenetic changes&mdash;their prognostic relevance and therapeutic potential merit further research in larger studies.</span></span></span></p> EZH2, SOCS1, Epigenetics, Methylation, Acute Myeloid Leukemia 310 318 http://journal.zums.ac.ir/browse.php?a_code=A-10-7270-1&slc_lang=en&sid=1 Farahnaz Saadat Gholami saadatfarahnaz@yahoo.com 5200319475328460085826 5200319475328460085826 No Department of Biology, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran Akbar Safipour Afshar asafshar4@gmail.com 5200319475328460085827 5200319475328460085827 No Department of Biology, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran Sajjad Sadeghi Zarghari SadeghiZS4012@mums.ac.ir 5200319475328460085828 5200319475328460085828 No Department of Hematology and Blood Banking, Mashhad University of Medical Sciences, Mashhad, Iran Arefeh Mazhari arefehmz1376@gmail.com 5200319475328460085829 5200319475328460085829 No Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Payam Siyadat payam.siyadat19899@gmail.com 5200319475328460085830 5200319475328460085830 No Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Maryam Sheikhi sheikhim2@mums.ac.ir 5200319475328460085831 5200319475328460085831 No Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Amirali Ayatollahi aytamirali@gmail.com 5200319475328460085832 5200319475328460085832 No Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Yasamin Ayatollahi yasaminayt@gmail.com 5200319475328460085833 5200319475328460085833 No Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Hossein Ayatollahi ayatollahih@mums.ac.ir 5200319475328460085834 5200319475328460085834 Yes Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran