en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <div style="text-align: justify;"><span style="font-size:14px;"><span style="line-height:2;"><span style="font-family:Times New Roman;"><strong>Background:</strong> The gingiva, a specialized part of the oral mucosa, is prone to frequent injury and exhibits a distinct wound healing process compared with other oral tissues. Caffeic acid phenethyl ester (CAPE), the primary bioactive compound in propolis, is known for its anti-inflammatory properties and its ability to enhance re-epithelialization. However, its role in modulating fibroblast activity and COL1A2 expression during gingival wound healing remains unexplored. Thus, the objective of this study was to investigate the COL1A2 expression in fibroblast underlying the effects of CAPE on gingival wound healing.<br> <strong>Materials and Methods: </strong>Forty-five male Balb/c mice were divided into three groups: control (saline), CAPE pre-treatment, and CAPE treatment. The CAPE was given through intraperitoneal (i.p) injection at a dose of 4 mg/kg body weight. Gingival lesions were created, and wound healing was assessed using clinical observations and histological analyses, including hematoxylin-eosin, toluidine blue, and immunohistochemistry COL1A2 staining, on days 0, 3, and 5.<br> <strong>Results:</strong> Histological analyses and clinical results revealed that CAPE administration significantly enhanced gingival wound closure in the pre-treatment and treatment groups compared with controls. The COL1A2 expression in fibroblast was notably increased in the CAPE-treated groups, suggesting accelerated fibroblast activity and tissue repair.<br> <strong>Conclusion:</strong> CAPE promotes gingival wound healing by enhancing fibroblast activity and COL1A2 expression, indicating its potential as a therapeutic agent for oral tissue repair.<span lang="EN-ID"><span style="font-family:&quot;Times New Roman&quot;,serif"></span></span></span></span></span><br> <br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri, sans-serif"><span style="color:#000000"><span style="font-style:normal"><span style="font-weight:400"><span style="white-space:normal"><span style="text-decoration:none"><span style="font-size:12pt"><span style="font-family:"Times New Roman", serif"></span></span></span></span></span></span></span></span></span></span></div> Caffeic Acid Phenethyl Ester, Fibroblasts, Wound Healing, Gingiva, Collagen Type I 52 52 http://journal.zums.ac.ir/browse.php?a_code=A-10-7281-1&slc_lang=en&sid=1 Yuli Berliani erlin.dentist@gmail.com 5200319475328460092201 5200319475328460092201 No Master Program of Dental Sciences (Oral Biology), Faculty of Dentistry, Universitas Islam Sultan Agung Islamy Rahma Hutami rahma.hutami@unissula.ac.id 5200319475328460092202 5200319475328460092202 Yes Department of Orthodontic, Faculty of Dentistry, Universitas Islam Sultan Agung Arin Oktaviani Suningdyastiningrum arin_oktaviani@udb.ac.id 5200319475328460092203 5200319475328460092203 No Department of Oral Biology, Faculty of Dentistry Universitas Duta Bangsa Sandy Christiono sandy@unissula.ac.id 5200319475328460092204 5200319475328460092204 No Department of Pediatric, Faculty of Dentistry, Universitas Islam Sultan Agung Erwid Fatchur Rahman erwid@unissula.ac.id 5200319475328460092205 5200319475328460092205 No Department of Oral Surgery, Faculty of Dentistry, Universitas Islam Sultan Agung, Semarang Arief Rahadian dr.ariefrahadian@gmail.com 5200319475328460092206 5200319475328460092206 No Department of Biochemical, Faculty of Medicine, Universitas Dian Nuswantoro Shaista Afroz shaista.pv@amu.ac.in 5200319475328460092207 5200319475328460092207 No Department of Prosthodontics/Dental Material, Dr. Ziauddin Ahmad Dental College, Aligarh Muslim University