en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#2d7f8f"><span style="color:white">Background & Objective:</span></span><b> </b></b><span style="color:black">Hyperprolactinemia is a common endocrine disorder characterized by elevated serum prolactin levels, which can result in menstrual irregularities, galactorrhea, infertility, and hypogonadism in women. Beyond its well-known reproductive consequences, emerging evidence suggests that hyperprolactinemia may also be associated with significant metabolic and inflammatory alterations. This study aimed to investigate the relationship between vitamin D (Vit D) deficiency and hormonal (follicle-stimulating hormone [FSH], estradiol, thyroid-stimulating hormone [TSH], triiodothyronine [T3], thyroxine [T4]) and biochemical parameters (insulin resistance, calcium, and C-reactive protein [CRP]) in women with hyperprolactinemia.</span><br> <b><span style="background:#2d7f8f"><span style="color:white"><span style="letter-spacing:-.1pt">&nbsp;Materials & Methods:</span></span></span></b> <span style="color:black"><span style="letter-spacing:-.1pt">The study included 40 women aged 30&ndash;40 years, divided into two groups: 20 women with hyperprolactinemia and 20 healthy controls. Hormonal and biochemical parameters were measured in both groups, and comparisons were made to assess the effect of vitamin D deficiency.</span></span><br> <b><span lang="EN-IN"><span style="background:#2d7f8f"><span style="color:white">Results: </span></span></span>&nbsp;</b><span style="color:black"><span style="letter-spacing:-.1pt">FSH levels increased in the hyperprolactinemia group but were not statistically significant, while estradiol showed a significant increase (<i>P</i> &le;0.01). TSH and T4 levels were also significantly elevated (<i>P</i> &le;0.01), whereas T3 increased significantly (<i>P</i> &le;0.05) compared with the control group. Insulin resistance (<i>P </i>&le;0.05) and CRP (<i>P </i>&le;0.01) were significantly higher in the hyperprolactinemic group. Serum calcium levels decreased significantly (<i>P</i> &le;0.05) compared with the control group.</span></span><br> <b><span style="background:#2d7f8f"><span style="color:white">Conclusion: </span></span>&nbsp;</b><span style="color:black">Vitamin D deficiency in hyperprolactinemic women may be associated with various hormonal and metabolic disturbances. These include alteration of reproductive and thyroid hormone levels and elevation of inflammatory markers, suggesting that vitamin D deficiency may contribute to broader endocrine and biochemical dysregulation.</span></span></span></p> Vitamin D Deficiency, Hyperprolactinemia, Insulin Resistance, CRP, Calcium 319 324 http://journal.zums.ac.ir/browse.php?a_code=A-10-7334-1&slc_lang=en&sid=1 Farah Kadhim Alwan farahka9624@gmail.com 5200319475328460086151 0009-0009-1763-4492 Yes Department of Biology, College of Science, University of Misan, Maysan, Iraq Ahmed Aboud Khalifa physiologist@uomisan.edu.iq 5200319475328460086152 5200319475328460086152 No Department of Biology, College of Science, University of Misan, Maysan, Iraq