en Life Science Life Science مقاله پژوهشی Original Research Article <span style="font-size:11pt"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:14.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Background and objectives: </span></span></span></b><span style="font-size:14.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Colorectal cancer (CRC) is the third most common diagnosis and the second most lethal malignancy worldwide. In CRC, similar to other malignancies, metastases are the leading cause of cancer-related mortality. Ziziphora clinopodioides belongs to the mint family (Lamiaceae) and shows the anticancer effect. The present study was conducted to investigate this plant extract&#39;s effects on CRC cells&#39; metastases power.</span></span></span><br> <b><span style="font-size:14.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></span></b><span style="font-size:14.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> The hydroalcoholic extract of Ziziphora clinopodioides was prepared. The effect of the extract on CRC cells (SW742) viability was tested using MTT assay. The IC50 values were calculated using GraphPad PRISM software. Cytotoxicity of the extract was measured by lactate dehydrogenase release assay. Then, cell migration, invasion, and adhesion assay were performed. Finally, gene expression analysis was done using Real-time PCR.</span></span></span><br> <b><span style="font-size:14.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b><span style="font-size:14.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> Treatment with extract reduced cell viability in a concentration- and time-dependent manner. The IC50 values of the extract were 760.59 &plusmn; 32.45, 395.16 &plusmn; 12.85, 241.87 &plusmn; 27.77, and 11.05 &plusmn; 11.05 for 24, 48, 72, and 96 hr. The migration, invasion, and adhesion potentials of the cells were significantly reduced after 24 hr treatment with the IC50 concentration of extract (p<0.05). Also, IC50 concentration of extract significantly decreased MMP-2, MMP-9, uPA, and uPAR expression (p<0.05), and significantly increased TIMP-2 expression after 24 hr treatment (P<0.05).</span></span></span><br> <b><span style="font-size:14.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span style="font-size:14.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> Hydroalcoholic extract of Ziziphora clinopodioides reduced the survival of CRC cells in a concentration- and time-dependent manner. It also showed antimetastatic activity and changed the expression of genes involved in cell metastasis.</span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br> &nbsp; Ziziphora clinopodioides, Migration, Invasion, Adhesion, Colorectal cancer 39 39 http://journal.zums.ac.ir/browse.php?a_code=A-10-7072-2&slc_lang=en&sid=1 Ali Pourmotaabed apourmotabbed@yahoo.com 5200319475328460090341 5200319475328460090341 Yes Department of Physiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran. Mona Pazhouhi mona.pazhouhi@gmail.com 5200319475328460090342 5200319475328460090342 No Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. Cyrus Jalili cjalili@gmail.com 5200319475328460090343 5200319475328460090343 No Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. Fuzieh Khani-Hemmatabadi fuziekhani@yahoo.com 5200319475328460090344 5200319475328460090344 No Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. Seyed Sharam Miraghaei shahram.miraghaee@yahoo.com 5200319475328460090345 5200319475328460090345 No Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. Mehrdad Ghanbari Mehrdadghanbari2002@yahoo.com 5200319475328460090346 5200319475328460090346 No Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran. Kazem Amini kazemamini1000@yahoo.com 5200319475328460090347 5200319475328460090347 No Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.