en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <span style="font-size:11pt"><span style="line-height:200%"><span calibri="" style="font-family:"><strong><span style="font-size:14.0pt"><span style="line-height:200%">Objective:</span></span></strong><strong> </strong><span new="" roman="" style="font-family:" times="">Metabolic syndrome (MS) is one of the most common disorders in post-menopausal women, which lacks effective, and safe treatment. The bioflavonoid of Hesperidin (Hsp) has beneficial effects in different disorders like cancer and cardiovascular disorders. </span><strong><span style="font-size:12.0pt"><span style="line-height:200%">This study aimed to investigate the interactive effect of supplementation with Hsp and 8-weeks moderate-intensity continuous training (MICT) on insulin resistance, body weight and dyslipidemia in a menopause-induced MS model in rats.</span></span></strong><strong><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-weight:normal"></span></span></span></strong></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span calibri="" style="font-family:"><strong><span style="font-size:14.0pt"><span style="line-height:200%">Methods:</span></span></strong><strong><span style="font-size:12.0pt"><span style="line-height:200%"> A total of 40 ovariectomized (OVX) Wistar rats were divided into five groups: control (OVX+Saline), exercise (Exe) (OVX + Exe), Hsp (Hsp) (OVX + Hsp), exercise and Hsp (OVX + Exe + Hsp), and sham-operated. </span></span></strong><span new="" roman="" style="font-family:" times="">Treatment included either intraperitoneal (i.p) injection of Hsp or saline injection, and also MICT training for 8 weeks. </span><span class="MsoPlaceholderText" style="color:#666666"><b>&nbsp;</b></span><strong><span style="font-size:12.0pt"><span style="line-height:200%">After the end of the treatment, body weight, body mass index (BMI), waist circumference (WC), visceral fat, and serum concentrations of glucose, insulin and lipid profile were measured using colorimetric analysis and ELISA.</span></span></strong><strong><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-weight:normal"></span></span></span></strong></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span calibri="" style="font-family:"><strong><span style="font-size:14.0pt"><span style="line-height:200%">Results:</span></span></strong><strong><span style="font-size:12.0pt"><span style="line-height:200%"> Data revealed a significant decrease in weight, waist circumference visceral fat, BMI and levels of glucose, homeostasis model assessment of insulin resistance, triacylglyceride, total cholesterol(p= 0.001), and low-density lipoprotein (LDL) (p= 0.004), but an increase in high-density lipoprotein (p= 0.001)), in OVX+Exe+Hsp compared with the OVX group.</span></span></strong><strong><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-weight:normal"></span></span></span></strong></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span calibri="" style="font-family:"><strong><span style="font-size:14.0pt"><span style="line-height:200%">Conclusions: The</span></span></strong><strong><span style="font-size:12.0pt"><span style="line-height:200%"> present study indicates that bioflavonoid Hsp combined with MICT effectively reduces body weight, visceral fat, and LDL, glucose, insulin resistance.</span></span></strong><strong><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-weight:normal"></span></span></span></strong></span></span></span><br> <br> &nbsp; Keywords: Menopouse, Metabolic Syndrome, Ovariectomy, MICT , Insulin resistance, Lipids, MS, Hsp 46 46 http://journal.zums.ac.ir/browse.php?a_code=A-10-7500-1&slc_lang=en&sid=1 Amirhossein Mohseni amir.hossein.m.12.81@gmail.com 5200319475328460090379 5200319475328460090379 No 1- Cellular & Molecular Research Center, Faculty of Medicine ,Guilan University of Medical Sciences, Rasht parvin babaei parvin.mma76@gmail.com 5200319475328460090378 5200319475328460090378 Yes 1- Cellular & Molecular Research Center, Faculty of Medicine ,Guilan University of Medical Sciences, Rasht Zeinab Bayat zeinabbayat93@gmail.com 5200319475328460090381 5200319475328460090381 No 4Dept. of Sport Physiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht ,Iran Aghil Esmaeili aghilesmaeili62@gmail.com 5200319475328460090380 5200319475328460090380 No Department of Medical Biotechnology, School of Paramedical Science, Guilan University of Medical Sciences, Rasht, Iran