Journal of Advances in Medical and Biomedical Research

en Associations of Sex Hormone-Binding Globulin Gene Polymorphisms and Circulating Levels with Metabolic Dysfunction in an Iraqi Population: A Case-Control Study Clinical Medicine Clinical Medicine مقاله پژوهشی Original Research Article Abstract Background One of the newly reported novel biomarkers is sex hormone-binding globulin (SHBG). Recently, it is considered as a potential predictor for type 2 diabetes mellitus (T2DM) and metabolic dysfunction. Polymorphism of SHBG gene may lead to changes in Serum SHBG levels which could lead to different metabolic conditions. This study aimed to investigate these associations in Iraqi population. Methods This was a case-control study by using 600 specimens. A quantitative enzyme-linked immunosorbent assay (ELISA) with other standardized laboratory techniques were used. By using TaqMan real-time polymerase chain reaction assays all genotyping for the polymorphisms [rs1799941 and rs6257] was performed. Chi-square test, multinomial logistic regression, two-way ANCOVA and ROC analysis was performed to assess statistical analysis. Results Serum SHBG concentrations steadily decreased along the metabolic continuum from healthy controls to individuals with IR and patients with T2DM (P < 0.001). After assessing genotype distributions, it was found that a significant differences (P < 0.001). rs1799941 AA and rs6257 CC genotypes were independently linked to T2DM susceptibility. Both polymorphisms independently significantly affected circulating SHBG concentrations (P = 4.7 × 10−12 and P = 0.03). SHBG demonstrated great discriminatory performance with area under the curve (AUC) of 0.928 (95% CI: 0.903–0.953) and this was used to differentiate T2DM from healthy controls Conclusions A significant association was observed between decreased circulating SHBG concentrations and metabolic changes or T2DM. Both concentrations of SHBG in serum and risk of T2DM are independently correlated with the SHBG polymorphisms rs1799941 and rs6257.   Sex hormone-binding globulin, rs1799941, rs6257, insulin resistance, type 2 diabetes mellitus. 12 12 http://journal.zums.ac.ir/browse.php?a_code=A-10-7417-2&slc_lang=en&sid=1 Hazim Hussein hazim.ali@jmu.edu.iq 5200319475328460090225 5200319475328460090225 Yes Jabir Ibn Hayyan University for Medical and Pharmaceutical Sciences, Iraq Mohammed Salman mohammed.noori@jmu.edu.iq 5200319475328460090226 5200319475328460090226 No Jabir Ibn Hayyan University for Medical and Pharmaceutical Sciences, Iraq Abdulhussein Algenabi aalhussein.aljanabi@uokufa.edu.iq 5200319475328460090227 5200319475328460090227 No Kufa University