fa تعیین گونه‌ی فاسیولا با استفاده از روش PCR-RFLP مقاله پژوهشی Original Research Article <p>Background and Objective: Fascioliasis is an important zoonotic disease that causes several health problems and economical losses in different parts of Iran including Zanjan. Fasciola hepatica and F. gigantica are recognized as causative agents of the disease. The differential diagnosis between these two species is very important for planning and control of infection. This study was designed to identify the Fasciola species by molecular methods in Zanjan (Iran). Methods and Materials: A number of 535 adult Fasciola worms were collected from the natural infected livers of cattles and sheep in local slaughterhouse. Living flukes were washed extensively in PBS at 37 OC and then anterior half of adult worms were stored at -20 OC in 70% ethanol. Total genomic DNA was extracted from individual flukes by modified phenol-chloroform method. Nucleotide polymorphism of ITS2 fragment of rDNA was investigated using PCR-RFLP assay and sequencing technique. Results: The results of PCR-RFLP and comparison of ITS2 sequences with the BLAST GenBank database clarified that all specimens were F. hepatica. The obtained sequences are available in the GenBank, with accession numbers EU391412 to EU391424. Conclusion: The results of this study showed no evidence of F. gigantica infection in sheep and cattles in Zanjan as all of the isolates were found to be F. hepatica. Key words: Fasciola, Liver flukes, rDNA, ITS2, PCR-RFLP</p> Key words: Fasciola, Liver flukes, rDNA, ITS2, PCR-RFLP 41 48 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-468&slc_lang=fa&sid=1 Pari Rahimi پری رحیمی 5200319475328460060597 5200319475328460060597 No Dept. of Parasitology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Mohammad Bagher Ghavami محمد باقر قوامی 5200319475328460060598 5200319475328460060598 No Dept. of Parasitology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Ali Haniloo علی هانیلو hani@zums.ac.ir 5200319475328460060599 5200319475328460060599 Yes Dept. of Parasitology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Abbas Ali Nourian عباسعلی نوریان 5200319475328460060600 5200319475328460060600 No Dept. of Parasitology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Alireza Biglari علیرضا بیگلری 5200319475328460060601 5200319475328460060601 No Dept. of Pathology and Mulecular Medicine, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran