Journal of Advances in Medical and Biomedical Research

fa بررسی فراوانی پلی‌مرفیسم‌های ژن گیرنده‌ی ویتامین D در مبتلایان به سل ریوی و ارتباط آن با تمایل به ابتلا The Frequency of Vitamin D Receptor Gene Polymorphisms in Pulmonary tuberculosis (TB) Patients and Its Relation with Susceptibility to TB مقاله پژوهشی Original Research Article <p>Background and Objective: Many genetic studies on predisposing factors for active tuberculosis have been conducted. Study on human leukocyte antigens (HLA), vitamin D receptor (VDR), NRAMP1, mannose binding lectin (MBL), and tumor necrotizing factor (TNF) are the most studies in this field. This study was planned to identify any relationship between VDR polymorphisms (Apa I, Bsm I, Fok I and Taq I) and susceptibility to TB. Materials and Methods: This case-control study was performed on blood samples from tuberculosis cases (n=164) and controls (n=50). DNA was extracted from white blood cells and the sequences were amplified by PCR followed by restriction digestion (PCR-RFLP technique) using specific primers and enzymes for each polymorphism. VDR polymorphisms were evaluated for two mentioned groups. Results: Two genotypes of AbfT and AabbFfTT were the only statistically significant genotypes which had a dfferent frequency between the study and control groups. Conclusion: Results of this study showed that genotypes of AbfT and AabbFfTT are protective factors against TB in our patients. We could not find any genotype as a predisposing factor for TB in our study group. However, other studies with larger group of samples are needed to find such a relationship.</p> Keywords: Pulmonary TB, Vitamin D receptor, Polymorphism 1 10 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-484&slc_lang=fa&sid=1 Seyed Mehran Marashian سید مهران مرعشیان mehranmarashian@gmail.com 5200319475328460060474 5200319475328460060474 Yes Mycobacteriology Research Center (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Masih Daneshvari Hospital, Tehran, Iran. Parisa Farnia پریسا فرنیا 5200319475328460060475 5200319475328460060475 No Mycobacteriology Research Center (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Masih Daneshvari Hospital, Tehran, Iran. SHima Seif شیما سیف 5200319475328460060476 5200319475328460060476 No Mycobacteriology Research Center (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Masih Daneshvari Hospital, Tehran, Iran. Saber Anoosheh صابر انوشه 5200319475328460060477 5200319475328460060477 No Mycobacteriology Research Center (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Masih Daneshvari Hospital, Tehran, Iran.