fa ارزیابی روش جدید برای تهیه‌ی گلبول قرمز شسته شده (سیستم بسته) در مقایسه با روش سنتی (سیستم باز) مقاله پژوهشی Original Research Article <p style="text-align: justify;"><strong><em>Background and Objective:</em></strong> Leukocyte filters are effective for WBC reduction but they cannot inhibit passing plasma proteins and as a result repeated protein entry may produce allergic transfusion reactions. To deal with this problem, washed RBC method is used. The traditional wash method is an open system through which waste products are carried away in sewers with the risk of environmental pollution. Newly introduced approach for washed RBCs consists of a closed system whereby waste products enter into a bag. In this study, the two methods were compared.<br> <strong><em>Materials and Methods:</em></strong> The two open and closed wash methods were compared in terms of health system, leukoreduction, risk of transmission of infection and quality control.&nbsp; In each method, 100 bags were washed, coded and then transmitted to different units of blood culture, flowcytometry as well as quality control. The data were collected and analyzed by SPSS14.<br> <a name="OLE_LINK56"></a><a name="OLE_LINK55"><strong><em>Results:</em></strong></a> 200 bags (100 for each method) were studied.&nbsp; Microbiologically, there were no positive results for any of the methods. In quality control also there was not any significant difference in the two methods. In flowcytometry, we didn&rsquo;t observe any significant correlation in leukocyte count in the two methods before washing (p=0.072), however. The correlation between them after washing (p<0.0001), demonstrating that the new method was better for leukoreduction.<br> <strong><em>Conclusion:</em></strong> The new washing system method was a superior way because it involves a closed system where waste products are discharged into a side bag and disposed as hospital waste. Meanwhile, this approach is more convenient for leukoreduction. In our country, since we still need a washing system for some transfusions, this method is deemed to be a decent and practical one because it impedes environmental pollution.</p> Blood transfusion, Washed RBCs, Leukoreduced component, Thalassemia 66 76 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-581&slc_lang=fa&sid=1 Azita Azarkeivan آزیتا آذرکیوان azazarkeivan@yahoo.com 5200319475328460049669 5200319475328460049669 Yes Thalassemia Clinic, Research Center of Iranian Blood Transfusion Organization, Tehran, Iran فوق تخصص هماتولوژی انکولوژی کودکان، استادیار موسسه عالی آموزشی و پژوهشی طب انتقال خون، مرکز تحقیقات سازمان انتقال خون ایران، درمانگاه تالاسمی Mohammad Hussein Arjangyan محمدحسین ارژنگیان 5200319475328460049670 5200319475328460049670 No Thalassemia Clinic, Research Center of Iranian Blood Transfusion Organization, Tehran, Iran کارشناس آزمایشگاه مرکز تحقیقات سازمان انتقال خون ایران، موسسه عالی آموزشی و پژوهشی طب انتقال خون، درمانگاه تالاسمی Bashir Hajibeigi بشیر حاجی‌بیگی 5200319475328460049671 5200319475328460049671 No Thalassemia Clinic, Research Center of Iranian Blood Transfusion Organization, Tehran, Iran پزشک، مرکز تحقیقات سازمان انتقال خون، موسسه عالی آموزشی و پژوهشی طب انتقال خون، درمانگاه تالاسمی Hojat Afradi حجت افرادی 5200319475328460049672 5200319475328460049672 No Thalassemia Clinic, Research Center of Iranian Blood Transfusion Organization, Tehran, Iran متخصص پاتولوژی، استادیار مرکز تحقیقات سازمان انتقال خون ایران، موسسه عالی آموزشی و پژوهشی طب انتقال خون، بخش فلوسیتومتری Mahnaz Aghaeepour مهناز آقائی‌پور 5200319475328460049673 5200319475328460049673 No Flowcytomery Section, Research Center of Iranian Blood Transfusion Organization, Tehran, Iran متخصص پاتولوژی، استادیار، مرکز تحقیقات سازمان انتقال خون ایران، موسسه عالی آموزشی و پژوهشی طب انتقال خون، بخش میکروب شناسی Farhad Razjoo فرهاد رازجو 5200319475328460049674 5200319475328460049674 No Microbiology Lab, Research Center of Iranian Blood Transfusion Organization, Tehran, Iran متخصص پاتولوژی، استادیار مرکز تحقیقات سازمان انتقال خون ایران، موسسه عالی آموزشی و پژوهشی طب انتقال خون، بخش کنترل کیفی Shahin Sharifi شهین شریفی 5200319475328460049675 5200319475328460049675 No Quality Control Lab, Research Center of Iranian Blood Transfusion Organization, Tehran, Iran فوق تخصص هماتولوژی کودکان، دانشیار دانشگاه علوم پزشکی شهید بهشتی Peyman Eshghi پیمان عشقی 5200319475328460049676 5200319475328460049676 No Mofid Children Hospital,Shahid Beheshti University of Medical Sciences, Tehran, Iran فوق تخصص هماتولوژی کودکان، دانشیار دانشگاه علوم پزشکی شهید بهشتی