Journal of Advances in Medical and Biomedical Research

fa فراوانی بتالاکتامازهای وسیع الطیف و گروه CTX-M-I در سویه‌های اشریشیا کلی جدا شده از عفونت‌های ادراری به دو روش فنوتیپی وPCR در خوی، ایران The Frequency of Extended Spectrum Beta Lactamase and CTX M-I of Escherichia Coli Isolated from the Urine Tract Infection of Patients by Phenotypic and PCR Methods in the City of Khoy in Iran مقاله پژوهشی Original Research Article Background and Objective: The production of Extended Spectrum Beta Lactamases (ESBLs) by Escherichia coli is the main cause of resistance to Cephalosporins.  In the past decade, CTX-M enzymes have become the most prevalent ESBLs in Europe, Canada, and Asia.  In this study, the frequency of ESBL-producing E.coli and molecular detection of the CTX-M-I group was investigated. Materials and Methods: A total of 400 urine samples were collected from both hospitalized and out-patients in Khoy’s hospitals between November 2009 and April 2010.  Out of these samples, 188 were identified as  E.coli  by standard biochemical tests. The antibiotic Susceptibility tests to 10 antibiotics were performed by the-disk-agar diffusion (DAD) method. ESBL production was screened by phenotypic test that including disk diffusion agar and combined disk as recommended by the Clinical and Laboratory Standards Institute (CLSI). Screened isolates were investigated by PCR assay for detection of CTX-M-I group genes. Results: The results show that out of 188 E.coli isolates identified, 56 (29.8%) were producing ESBls by phenotypic test. All isolates were sensitive to imipenem. Overall, 49 (87.5%) isolates were confirmed as CTX-M-I producer by PCR. Conclusion: The results of this study showed that about 30% of the identified E.coli were producing ESBl. Therefore, we recommend to use molecular methods in such researches. E.coli, Extended Spectrum Beta Lactamase, CTX-M-I, Antimicrobial resistance 53 61 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-605&slc_lang=fa&sid=1 Mohammad Kazem Sharifi yazdi محمد کاظم شریفی یزدی 5200319475328460060079 5200319475328460060079 No Dept. of Laboratory, Faculty Paramedical Sciences, Tehran University of Medical Sciences. Tehran, Iran Mohammad Azarsa محمد آذرسا 5200319475328460060080 5200319475328460060080 No Devision of Microbiology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Hasan Shirazi محمدحسن شیرازی 5200319475328460060081 5200319475328460060081 No Antimicrobial Resistant Research Center, Tehran University of Medical Sciences, Tehran Iran Abdolaziz Rastegar Lari عبدالعزیز رستگاری لاری 5200319475328460060082 5200319475328460060082 No Dept. of Medical Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran P Owlia پرویز اولیا 5200319475328460060083 5200319475328460060083 No Dept. of Medical Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran . J fallah Mehrabadi جلیل فلاح مهر آبادی 5200319475328460060084 5200319475328460060084 No MARS Bioinformatics Institute, Tehran, Iran. Hedroosha Molla Aghamirzaei هدروشا ملا آقا میرزایی 5200319475328460060085 5200319475328460060085 No Devision of Microbiology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Ailar Sabbaghi آیلار صباغی 5200319475328460060086 5200319475328460060086 No Devision of Microbiology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Farnaz Shamkani فرناز شامکانی 5200319475328460060087 5200319475328460060087 No Dept. of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, International Units(ARAS), Iran Golnar Mobasseri گلناز مبصری 5200319475328460060088 5200319475328460060088 No Devision of Microbiology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Ronak Bakhtiari روناک بختیاری 5200319475328460060089 5200319475328460060089 No Devision of Microbiology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Mahdi Soltan Dallal محمدمهدی سلطان دلال soltanirad34@yahoo.com 5200319475328460060090 5200319475328460060090 Yes Devision of Microbiology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.