fa کلونینگ و بیان دومین انتهای آمینی فلاژلین پسودوموناس آئروژینوزا و ارزیابی آنتی‌بادی‌های تولید شده بر علیه آن در مهار حرکت پسودوموناس آئروژینوزا کارآزمایی بالینی Clinical Trials <p>Background and Objective: Pseudomonas aeruginosa is an opportunistic pathogen that causes severe and lethal infections in immunocompromised individuals. This bacterium possesses a single polar flagellum. Flagellum and its subunit Flagellin play important roles in the pathogenesis of P. aeruginosa. Flagellin induces immune responses by interaction of its N-terminal domain with TLR-5. Our main aims of this study were cloning and expression of N-terminal domains of flagellin and evaluation of antibodies raised against it on motility inhibition of P. aeruginosa. Material and Methods: The DNA sequence coding for the first 161 amino acids of flagellin was PCR amplified and cloned into a pET-28a expression vector. Recombinant protein was over expressed in BL-21(DE3), and purified by Ni-NTA resin. The immune reactivity of recombinant truncated flagellin was evaluated by Western blotting. The recombinant protein was injected into a rabbit and antibodies raised against it were evaluated for the cell motility inhibition of P. aeruginosa 8821M. Results: The N-terminal domain of Flagellin was successfully overexpressed in Escherichia coli BL-21(DE3) host strain. Anti-native and anti-N-terminal flagellin antibodies reacted with the recombinant protein. Motility inhibition assay demonstrated that polyclonal antiserum against N-teminal flagellin is able to inhibit the motility of P. aeruginosa 8821M. Conclusion: The N-terminal domain of flagellin may be used for development of a new recombinant vaccine against P. aeruginosa infections.</p> Keywords: Cloning, Motility inhibition, N-terminal domains of flagellin, Pseudomonas aeruginosa 1 11 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-636&slc_lang=fa&sid=1 Farideh Dakterzada فریده دکتر زاده 5200319475328460059850 5200319475328460059850 No Dept. of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Ashraf Mohabati Mobarez اشرف محبتی مبارز mmmobarez@modares.ac.ir 5200319475328460059851 5200319475328460059851 Yes Dept. of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Mahyar Habibi Roudkenar مهریار حبیبی رود کنار 5200319475328460059852 5200319475328460059852 No Research Center, Iranian Blood Transfusion Organization, Tehran, Iran Mehdi Forouzandeh مهدی فروزنده مقدم 5200319475328460059853 5200319475328460059853 No Dept. of Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.