fa بررسی اثر فتودینامیکی کمپلکس تتراپیریدینو پورفیرازین روی (II)، بر رده‌ی سلولی HeLa کارآزمایی بالینی Clinical Trials <p>Background and Objective: Photodynamic therapy is a treatment that uses photosensitizer and intense visible light. When photosensitizers get exposed to a specific light wavelength (preferentially in the red region), they produce reactive oxygen species that are toxic to cells. Recently, attention has been focused on porphyrins and their analogs as photosensitizers. Zn (II) tetrapyridinoporphyrazin complex is a water-soluble photosensitizer that has a good potential for application in photodynamic therapy. In this study, phototoxic effect of this complex on HeLa cancer cell line has been investigated. Materials and Methods: HeLa cell cultures were treated with different concentrations of Zn (II) tetrapyridinoporphyrazin. The cytotoxic effects were measured both in the presence and absence of light using the MTT assay. The light source was a 150W tungsten halogen lamp equipped with a red filter. Results: Our data indicate that porphyrazine&rsquo;s photocytotoxicity is remarkably more significant than its cytotoxycity in the dark. Statistical analysis showed the effective dose (ED50) values in the dark and light conditions were 8.6 and 4.2 &micro;M, respectively. In addition, the results imply that in the range of 0-12 &micro;M, the increase in the complex concentration correlates with the increase in the cytotoxicity effect. However, the cytotoxicity decreases at the higher concentration (50&micro;M), which is likely due to aggregation of the complex. Conclusion: Our results show that Zn (II) tetrapyridinoporphyrazin complex may be a promising photosensitizer for innovative photodynamic therapy and may have a high potential application in cancer treatment. Furthermore, it seems to have more benefits compared to other known photosensitizers.</p> Keywords: Zn(II)tetrapyridinoporphyrazin, Photodynamic therapy, Photosensitizer, HeLa cell line, MTT assay 51 63 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-641&slc_lang=fa&sid=1 Farzaneh Fakheri فرزانه فاخری 5200319475328460059894 5200319475328460059894 No Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Hosein Rastegar حسین رستگار mhrastegar@yahoo.com 5200319475328460059895 5200319475328460059895 Yes Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Mahmoud Alebouyeh محمود آل بویه 5200319475328460059896 5200319475328460059896 No Food and Drug Control Research Center, FDCL MOH, Ministry of Health and Medical Education, Tehran, Iran. Abdoreza Esmaeilzadeh عبدالرضا اسماعیل‌زاده 5200319475328460059897 5200319475328460059897 No Dept. of Immunology, Medical Faculty, Zanjan University of Medical Sciences, Zanjan, Iran. Seyed Esmaeil Balaghi سید اسماعیل بلاغی 5200319475328460059898 5200319475328460059898 No Dept. of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, Iran Fatemeh Hakimian فاطمه حکیمیان 5200319475328460059899 5200319475328460059899 No Dept. of Biological sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, Iran Mino Akbari مینو اکبری 5200319475328460059900 5200319475328460059900 No Food and Drug Control Research Center, FDCL MOH, Ministry of Health and Medical Education, Tehran, Iran. Seyed Shahab Mousavi Motlagh سید شهاب موسوی مطلق 5200319475328460059901 5200319475328460059901 No Food and Drug Control Research Center, FDCL MOH, Ministry of Health and Medical Education, Tehran, Iran.