fa اثرات عصاره‌ی آبی الکلی گیاهان بیلهر، زنیان و سنبله کرکدار بر تکثیر الیگودندروسیت‌های مغز موش صحرایی کارآزمایی بالینی Clinical Trials <p>Background and Objective: Dorema aucheri, Stachys pilifera and Trachyspermum copticum are used in Iranian traditional medicine especially in the province of Kohgilouyeh and Boyerahmad. This study aimed to determine the effect of these plants on proliferation of rat brain oligodendrocytes. Materials and Methods: Macerated hydroalcoholic extract of each plant was prepared using 50% ethanol. The OLN-93 oligodendrocytes were incubated for 24 h with different concentrations of these extracts. Then, percentage of viable cells was determined using a method based on the reduction of tetrazolium salt. Results: Extract of Dorema aucheri at 50, 100, 200, and 400 &micro;g/ml decreased viable cells from 100 &plusmn; 4% (control cells) to 84 &plusmn; 4, 58 &plusmn; 6 (P<0.001), 6 &plusmn; 0.5 (P<0.001), 4 &plusmn; 0.5% (P<0.001), respectively. Similarly, Stachys pilifera extract at 50, 100, 200 and 400 &micro;g/ml decreased viable cells from 100 &plusmn; 4% to 81 &plusmn; 6, 64 &plusmn; 3 (P<0.01), 61 &plusmn; 4 (P<0.01), 55 &plusmn; 2% (P<0.001), respectively. Also, Trachyspermum copticum extract at concentrations higher than 50 &micro;g/ml significantly decreased percentage of viable cells. Conclusion: Extracts of Dorema aucheri, Trachyspermum copticum and Stachys pilifera reduce proliferation of rat brain oligodendrocytes.</p> Keywords: Dorema aucheri boiss, Stachys pilifera, Trachyspermum copticum, Oln-93 cell line, Antiproliferative effect 59 66 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-830&slc_lang=fa&sid=1 Seyed Mohsen Mortazavian سید محسن مرتضویان 5200319475328460059130 5200319475328460059130 No Dept. of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 2Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Zahra Hosainian زهرا حسینیان 5200319475328460059131 5200319475328460059131 No Dept. of Neurology, Zanjan University of Medical Sciences and Health Services, Zanjan, Iran. Ahmad Ghorbani احمد قربانی ghorbania@mums.ac.ir 5200319475328460059132 5200319475328460059132 Yes Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.