fa بررسی اثر کشندگی پپتید بروینین-2R بر سلول‌های اسکواموس کارسینوم دهانی کارآزمایی بالینی Clinical Trials <p><strong><em>Background and Objective:</em></strong> Peptide as an agent in manufacturing new drugs has recently been taken into consideration. Brevinin-2R is a nano peptide with a wide range of biological actions. Thus, the current study was designed in order to evaluate the in vitro effects of Brevinin-2R on oral squamous cell carcinoma cells in comparison to normal mice fibroblast cells.</p> <p><strong><em>Materials and Methods:</em></strong> In this study, oral squamous cell carcinoma cells (KB) and normal mice fibroblast cells (L929) were cultured and&nbsp; treated with different concentrations of Brevinin-2R (0.1-30 &micro;g/ml) for 24, 48 and 72 hours. Then, cell viability was calculated by MTT method. Data were fed to SPSS software (version 20) via which One Way ANOVA and Tukey tests was calculated for the collected data.</p> <p><strong><em>Results:</em></strong> The half-maximal inhibitory concentration (IC50) of Brevinin-2R for KB cells and L929 cells were 15.48 and 24.14 &micro;g/ml respectively, demonstrating that higher concentration of Brevinin-2R is necessary in order to reach IC50 concentration for L929 cells (about 1.5 times).</p> <p><strong><em>Conclusion:</em></strong> Results point out that Brevinin-2R peptide is more effective on oral squamous cell carcinoma cells compared to normal mice fibroblast cells (L929).</p> <p></p><p></p> Keywords: Brevinin-2R, Cell cytotoxic, Oral squamous cell carcinoma, Medical peptide 39 48 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-974&slc_lang=fa&sid=1 Faezeh Khazimeh فائزه خزیمه 5200319475328460053323 5200319475328460053323 No Dental Research Center, Isfahan University of Medical Sciences, Isfahan, Iran دانشگاه علوم پزشکی اصفهان Zahra Golestan Nezhad زهرا گلستان نژاد 5200319475328460053324 5200319475328460053324 No Dental Research Center, Isfahan University of Medical Sciences, Isfahan, Iran دانشگاه علوم پزشکی اصفهان Azizollah Bakhtari عزیزالله باختری 5200319475328460053325 5200319475328460053325 No Dept. of Animal Sciences, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran دانشگاه صنعتی اصفهان Mahin Bakhshi مهین بخشی 5200319475328460053326 5200319475328460053326 No Dept. of Dental Diseases, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran دانشگاه علوم پزشکی شهید بهشتی تهران Hazhir Yousef Shahi هژیر یوسف شاهی 5200319475328460053327 5200319475328460053327 No Student Research Committee, Faculty of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran دانشگاه علوم پزشکی اصفهان Shahin Gavanji شاهین گوانجی 5200319475328460053328 5200319475328460053328 Yes Young Researchers and Elite Club, Islamic Azad University, Isfahan (khorasgan) Branch, Isfahan, Iran دانشگاه آزاد اسلامی واحد خوراسگان