fa تعیین گروه‌های فیلوژنتیک و مقاومت آنتی‌بیوتیکی در ایزوله‌های اشریشیاکلی اوروپاتوژنیک و کامنسال جدا شده از شهر زنجان کارآزمایی بالینی Clinical Trials <p><strong><em>Background and Objective:</em></strong> <em>Escherichia coli </em>strains are the major cause of urinary tract infections which belong to the large group of extra-intestinal pathogenic <em>E. coli</em>. They fall into four main phylogenetic groups: A, B1, B2 and D. The aim of the present study was to determine phylogenetic groups in uropathogenic and commensal <em>Escherichia coli </em>isolated. Also, due to increase in the rate of antibiotic usage and subsequent drug resistance, this study evaluated the antimicrobial resistance pattern of <em>E. coli </em>isolated from patients with UTI.</p> <p><strong><em>Materials and Methods</em></strong><strong>:</strong> In this cross-sectional study, 137 uropathogenic<em> Escherichia coli </em>isolates were collected from the clinical specimens of Zanjan hospitals and 50 isolates were collected from healthy adults. After verifying isolates via biochemical methods and extraction of total DNA, Multiplex <a name="OLE_LINK6"></a><a name="OLE_LINK5">PCR</a> was done by specific primers for phylogenetic grouping. The antibiotic susceptibility test (disk diffusion method) was done according to <a name="OLE_LINK4"></a><a name="OLE_LINK3">CLSI</a> advice against 13 antibiotics.</p> <p><strong><em>Results</em></strong><strong>:</strong> In this study, the highest rates of resistance to antibiotics in UTI isolates were seen against ampicillin (74.5%) and aztreonam (59.1%). Also, the lowest rates of resistance were reported against imipenem (1.5%) and amikacin (10.9%).The distribution of UPEC phy logenetic groups typing marked the highest prevalence in group B2 (67.15 %) , and then in group D (21.17%) and the lowest prevalence in group A (11.68%). Phy logenetic B1 was not observed in uropathogenic isolates. Among the commensal isolates, 52% belonged to phylogenetic group D, 24% to B2, 14% to A, and10% to B1.</p> <p><strong><em>Conclusion</em></strong><strong>:</strong> The results indicate the higher prevalence of B2 and D phylogenetic groups in commensal and uropathogenic<em> Escherichia coli</em> strains in Zanjan<strong>.</strong></p> <p></p><p></p> Keywords: Uropathogenic escherichia coli, Commensal, Phylogenrtic groups, Antibiotic resistance, PCR 107 118 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-980&slc_lang=fa&sid=1 Roya Sohrabi رویا سهرابی 5200319475328460053377 5200319475328460053377 No Dept. of Microbiology, Islamic Azad University Zanjan Branch, Zanjan, Iran دانشگاه آزاد اسلامی واحد زنجان Habib Zeighami حبیب ضیغمی zeighami@zums.ac.ir 5200319475328460053378 5200319475328460053378 Yes Dept. of Microbiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشگاه علوم پزشکی زنجان