fa بررسی اثر مهارکننده‌ی کربونیل سیانید 3-کلروفنیل هیدرازون و تعیین میزان بیان پمپ ترشحی MexCD-oprJبر روی ایزوله‌های سودوموناس آئروژینوزا جدا شده از بیماران بستری در بیمارستان شهید مطهری شهر تهران کارآزمایی بالینی Clinical Trials <p><strong><em>Background</em></strong><em> <strong>and Objective:</strong></em> Efflux pumps are one of the major resistance mechanisms in <em>Pseudomonas aeruginosa </em>that inhibit the accumulation of antibiotics in bacterial cells. The aim of this study was determining the effect of <a href="https://en.wikipedia.org/wiki/Carbonyl_cyanide_m-chlorophenyl_hydrazone">carbonyl cyanide 3-chlorophenyl hydrazone</a> (CCCP) and detecting the overexpression of pump MexCD-oprJ in <em>P. aeruginosa</em> isolated from burn patients in Shahid Motahari hospital during 2014 and 2015.</p> <p><strong><em>Materials and Methods:</em></strong> This descriptive study was performed on 100<em> P. aeruginosa</em> isolates from burn patients in Shahid Motahari hospital. Antibiotic susceptibility tests were performed by Kirby-Bauer disc diffusion and broth microdilution methods according to the CLSI guidelines. The effect of CCCP was determined using the broth microdilution method. The expression levels of MexD-oprJ (an efflux pump) was evaluated by Real-Time PCR and finally detection of <em>nfxB</em> mutations was carried out by PCR and sequencing techniques.&nbsp;</p> <p><strong><em>Results:</em></strong> Antibiogram for 100 <em>P</em><em>. aeruginosa </em>showed maximum susceptibility and resistance to colistin (100%) and ticarcilin (98%), respectively. The inhibitory effect of CCCP was apparently seen in 16 isolates. Real-time PCR results for these strains showed overexpression in range of 0.26-15.34 (mean: 4/1). The PCR and sequencing techniques showed mutations that converts lucin to prolin in position 14 of the protein sequence.</p> <p><strong><em>Conclusion: </em></strong>The increase in expression level of efflux pump MexCD-oprJ shows the prevalence of <em>nfxB</em> mutants in burn wound infections. Therefore, use of <a href="https://en.wikipedia.org/wiki/Carbonyl_cyanide_m-chlorophenyl_hydrazone">carbonyl cyanide 3-chlorophenyl hydrazone</a> (CCCP) can help disrupt the function of efflux pumps and improve the effects of antibiotics.</p> <p></p><p></p> Keywords: Pseudomonas aeruginosa, Carbonyl cyanide 3-chlorophenyl hydrazone, Antibiotic resistance, MexCD-oprJ, Real time PCR 43 55 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-987&slc_lang=fa&sid=1 Samira Tarashi سمیرا تراشی 5200319475328460053629 5200319475328460053629 No Dept. of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran دانشگاه علوم پزشکی شهید بهشتی Hossein Goudarzi حسین گودرزی 5200319475328460053630 5200319475328460053630 No Dept. of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran دانشگاه علوم پزشکی شهید بهشتی Ali Hashemi علی هاشمی 5200319475328460053631 5200319475328460053631 Yes Dept. of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran دانشگاه علوم پزشکی شهید بهشتی Neda Yousefi Nojokambri ندا یوسفی نوجوکامبری 5200319475328460053632 5200319475328460053632 No Dept. of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran دانشگاه علوم پزشکی شهید بهشتی Mehrzad Sadreddin Amin مهرزاد صدرالدین امین 5200319475328460053633 5200319475328460053633 No Dept. of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran دانشگاه علوم پزشکی شهید بهشتی Elaheh Taki الهه تاکی 5200319475328460053634 5200319475328460053634 No Dept. of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran دانشگاه علوم پزشکی شهید بهشتی Mahboubeh Satarzadeh محبوبه ستارزاده 5200319475328460053635 5200319475328460053635 No Shahid Motahari Burning Hospital,Tehran,Iran بیمارستان سوانح و سوختگی شهید مطهری تهران