fa اثر نانو ذرات تیتانیوم دی اکسید ناخالص شده با نیتروژن بر رده‌ی سلول سرطانی A-375 ملانومای انسانی کارآزمایی بالینی Clinical Trials <p style="text-align: justify;"><strong><em>Backgrounds and Objective:</em></strong> Titanium dioxide (TiO₂) is a potent photosensitizer in photodynamic therapy (PDT) and is activated upon ultraviolet (UV) irradiation.The aim of this study was the assessment of the effect of nitrogen &ndash;nano doped of titanium dioxide in human A-375 melanoma cancer cell line.</p> <p style="text-align: justify;"><strong><em>Materials and Methods:</em></strong> N-TiO₂ nanoparticles were prepared by mechanical mixing of urea with TiO₂ powder in a 4:1 weight ratio. A-375 cells were treated with different concentrations of N-TiO₂ for various time intervals in the presence or absence of visible light. The growth and viability of A-375 cells were determined by means of trypan blue exclusion test and MTT assay, respectively. Acridine orange/Ethidium bromide double staining and DNA gel electrophoresis were applied to determine apoptosis.</p> <p style="text-align: justify;"><strong><em>Results: </em></strong>The results showed that visible light activated N-TiO₂ could induce growth inhibition in a dose (0.01-100 &mu;g/ml ) and time dependent manner. In concentration of 0.5 &mu;g/ml a significant difference was observed between the treated and the control samples.Decrease in viability was observed in 100 &mu;g/ml concentration and the results of fluorescent microscopy showed &nbsp;DNA fragmentation due to apoptotic cell <a>death</a><a href="#_msocom_1" id="_anchor_1" name="_msoanchor_1">[G1]</a>&nbsp; at this concentration.</p> <p><strong><em>Conclusion:</em></strong> Based on the anti-cancer effects of N-TiO₂ on A-375 cells, further evaluation of these nanoparticles as a new photosensitizer in photocatalytic therapy of skin cancer can be proposed.</p> <p></p><p></p> <div> <hr align="left" size="1" width="33%" > <div><div id="_com_1"></div></div> </div> Keywords: Melanoma, Nitrogen doping, Photodynamic therapy, Titanium dioxide 68 80 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-988&slc_lang=fa&sid=1 Maryam Sharifi مریم شریفی 5200319475328460053642 5200319475328460053642 No Dept. of Cell & Molecular Biology, Islamic Azad University Pharmaceutical Sciences Branch, Tehran, Iran دانشگاه آزاد اسلامی تهران Seyed Mohammad Mousavi سید محمد امین موسوی 5200319475328460053643 5200319475328460053643 Yes Division of Medical Biotechnology, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری تهران Tahereh Naji طاهره ناجی 5200319475328460053644 5200319475328460053644 No Dept. of Cell & Molecular Biology, Islamic Azad University Pharmaceutical Sciences Branch, Tehran, Iran دانشگاه آزاد اسلامی تهران