fa بررسی اثرات نیتریت سدیم برمیزان نیتریک اکساید و آنزیم‌های کبدی سرم و تغییرات هیستوپاتولوژیکی بافت کبد در موش‌های صحرایی نر بالغ کارآزمایی بالینی Clinical Trials <p><strong><em>Background and Objective:</em></strong> Nitrite is widely used in food, pharmaceutical and chemical products. This study was performed to examine the effects of sodium nitrite on liver histopathology and enzyme and nitric oxide in adult Wistar male rats.</p> <p><strong><em>Materials and Methods</em></strong><strong>:</strong> In this experimental study, 30 adult male Wistar rats were randomly divided into three experimental groups of 10, consisting of one control group and two experimental groups (one group receiving 100 mg/kg/day and the other 300 mg/kg/day of sodium nitrite in drinking water for 60 days). Blood samples were collected to determine serum levels of AST, ALT, ALP and nitric oxide. After sacrifying the rats, the rat&rsquo;s liver tissues were removed for histopathological evaluation (using Hematoxylin-Eosin staining). The obtained results were analyzed statistically.</p> <p><strong><em>Results:</em></strong> Use of sodium nitrite in both experimental groups caused a significant increase in serum AST, ALT, ALP and nitric oxide compared to the control group<span dir="RTL">) </span>p <span dir="RTL">&ge;</span>0.0<span dir="RTL"> (</span>. In the group receiving 300 mg/kg/day of sodium nitrite, dilatation and congestion of hepatic sinusoids with infiltration of mononuclear inflammatory cells in addition to sinusoid cell proliferation was observed.</p> <p><strong><em>Conclusion:</em></strong> Sodium nitrite increases nitric oxide levels and damages hepatocytes, therefore it is a risk factor for liver disease and should be replaced with a suitable and healthy substance in the food industry.</p> <p align="right" dir="RTL"></p><p></p> Keywords: Histopathology, Nitrite, Liver Enzyme, Nitric oxide, Rat 109 119 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1002&slc_lang=fa&sid=1 Fatemah Juibar فاطمه جویبار dr.f.juibar@gmail.com 5200319475328460053450 5200319475328460053450 Yes Young Researchers and Elite Club, Kazerun Branch, Islamic Azad University, Kazerun, Iran دانشگاه آزاد اسلامی واحد کازرون Hossein Fattahi حسین فتاحی 5200319475328460053451 5200319475328460053451 No Dept. of Microbiology, Islamic Azad University, Kazerun Branch, Kazerun, Iran دانشگاه آزاد اسلامی واحد کازرون Farshad Sadaty فرشاد ساداتی 5200319475328460053452 5200319475328460053452 No Dept. of Vterinary, Young Researchers and Elite Club, Kazerun Branch, Islamic Azad University, Kazerun, Iran دانشگاه آزاد اسلامی واحد کازرون