en مطالعه تأثیر پرتوسکیا آبرتانوئیدهای کارل بر سوختگی پوست در موش‌های نر: یافته های in-vivo و in-vitro Clinical Medicine Clinical Medicine مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-family:times new roman;"><span style="font-size:14px;"><span style="color:#008080;"><strong>Background & Objective:</strong></span> <em>Perovskia abrotanoides</em> Karel locally known as Brazambal, is widely used in medical practice to treat various disorders. Burn is a common source of injury and Brazambal ointment has been extensively used in burn wound healing in North of Iran. The purpose of this study was to examine the healing effect of Brazambal ointment in experimental model of burn. In addition, in-vitro healing characteristics and antibacterial activity of <em>P. abrotanoides</em> was investigated.<br> <span style="color:#008080;"><strong>Materials & Methods:</strong></span> Scald burn injury was produced by pouring 2.5cc of boiling water (95&ordm;C) on the posterior surface of rat&rsquo;s neck for 10 seconds. Twenty-eight adult male Sprague Dawley rats were divided into 4 groups (7 rats each) and followed for 7 days. Control group (no treatment); Silver sulfadiazine-treated group; Brazambal ointment-treated group; and vehicle-treated group received the complete ointment without Brazambal part. The effects of Brazambal aqueous extract on fibroblast cells and antibacterial activity against <em>Escherichia coli</em>, <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em> were evaluated.<br> <span style="color:#008080;"><strong>Results: </strong></span>Topical application of Brazambal ointment can accelerate wound healing process by initiating re-epithelialization and formation of granulation tissue. Proliferative activity of the aqueous extract on fibroblast cells was observed after 48 h in concentrations of 7.5% and 10% (<em>P</em><0.0001). However, <em>P<span dir="RTL">.</span> abrotanoides</em> Karel extract had no significant antibacterial effect.<br> <span style="color:#008080;"><strong>Conclusion: </strong></span>The presence of hair follicles, re-epithelialization and granulation tissue is suggestive of Brazambal ointment relative effectiveness on experimental scald burn in rats. However, further studies are warranted to reveal the healing mechanisms of <em>P. abrotanoides</em>.</span></span></p> Perovskia abrotanoides, Brazambal, Traditional medicine, Burn 17 22 http://journal.zums.ac.ir/browse.php?a_code=A-10-4579-1&slc_lang=en&sid=1 Amin Derakhshanfar امین درخشان‌فر aderakhshanfar@yahoo.com 5200319475328460054642 5200319475328460054642 No Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran Seyedeh-Sara Hashemi سیدسارا هاشمی sara_hashemi@sums.ac.ir 5200319475328460054643 5200319475328460054643 Yes Burn and Wound Healing Research Center, Shiraz University of Medical Science, Shiraz, Iran Javad Moayedi جواد موئیدی javad.moayedi88@gmail.com 5200319475328460054644 5200319475328460054644 No Burn and Wound Healing Research Center, Shiraz University of Medical Science, Shiraz, Iran Mahjoob Vahedi محجوب واحدی mahjoobvahedi60@gmail.com 5200319475328460054645 5200319475328460054645 No Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Davood Mehrabani داود مهربانی davood_mehrabani@yahoo.com 5200319475328460054646 5200319475328460054646 No Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz,Iran Abuzar Valizadeh ابوذر ولی‌زاده avalizadeh1358@gmail.com 5200319475328460054647 5200319475328460054647 No Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran