en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-family:times new roman;"><span style="font-size:16px;"><span style="color:#008080;"><strong>Background & Objective: </strong></span>Busulfan (BUS) as a drug for cancer treatment can produce free radicals. Crocin (CRO) is a potent antioxidant with beneficial effects. This study was designed to evaluate the effects of CRO against BUS-related damages to the male rats reproductive parameters.<br> <span style="color:#008080;"><strong>Materials & Methods:</strong></span> Forty-eight male rats were randomly assigned into 8 groups; Group 1 (normal control group) received normal saline; Group 2 (BUS control group) received 10 mg/kg/body weight (b.w.) of BUS; Group 3-5 (CRO groups) received 12.5, 25.0, 50.0 mg/kg/b.w. of CRO, respectively; Group 6-8 (BUS + CRO groups) received BUS + CRO. Treatments were administered daily by intraperitoneal injection in a five-week period. The sperm parameters, testis malondialdehyde (MDA), total antioxidant capacity (TAC), testosterone level, and germinal layer height were evaluated histologically and analyzed statistically.<br> <span style="color:#008080;"><strong>Results:</strong></span> All parameters reduced significantly in the BUS control group compared to the normal control group (<em>P</em><0.01) except the MDA level (which increased). The CRO and CRO + BUS treatments in all doses increased whole parameters significantly compared to the BUS control group (<em>P</em><0.01) except MDA level (which decreased). No significant modifications were observed in all CRO groups compared to the normal control group (<em>P</em>>0.05).<br> <span style="color:#008080;"><strong>Conclusion: </strong></span>It seems that CRO could increase the quality of spermatozoa and improves BUS -induced adverse effects on male fertility parameters.</span></span></p> Busulfan, Crocin, Fertility 25 33 http://journal.zums.ac.ir/browse.php?a_code=A-10-5165-1&slc_lang=en&sid=1 Shiva Roshankhah roshankhah@yahoo.com 5200319475328460052936 5200319475328460052936 No Dept. of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Amir Abdolmaleki enjoyanatomy@gmail.com 5200319475328460052937 5200319475328460052937 No Dept. of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Cyrus Jalili cjalili@yahoo.com 5200319475328460052938 5200319475328460052938 No Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran Mohammad Reza Salahshoor reza.salahshoor@yahoo.com 5200319475328460052939 5200319475328460052939 Yes Dept. of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran