en Pharmacology Pharmacology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span> </strong>Methylphenidate (MPH), as a central nervous system stimulant, is often used to manage hyperactive disorders. The literature is scarce regarding the behavioral consequences of chronic MPH treatment and the role of involved receptors. Thus, in the current study involved receptors in MPH induced-anxiety, depression and motor activity disorders were evaluated.</span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span> </strong>Seventy rats were randomly distributed to seven groups (n = 10, per group); they were treated with normal saline, Domoic acid (DOM) (as AMPA/kainite receptor agonist), <a href="https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwjW5PaojafKAhXn83IKHYU_BYEQFggfMAA&url=https%3A%2F%2Fen.wikipedia.org%2Fwiki%2FBicuculline&usg=AFQjCNGumiJhFR6dD2nYin_gPR6zaKvr6A&sig2=c1fCXc9QKNaXz7uP1Iw4mw&bvm=bv.111396085,d.bGQ">Bicuculline</a> (BIC) (as GABA_A receptor antagonist), Ketamine (KET) (as NMDA receptor antagonist ), Yohimbine (YOH) (as Alpha-2 adrenergic receptor antagonist), or Haloperidole (HAL) (as D₂dopamine receptor antagonist), simultaneously with MPH (10 mg/kg). On days 11, 17 and 24, some standard behavior tests including Elevated Plus Maze (EPM), Forced Swim Test (FST) and Open Field Test (OFT) were used to investigate anxiety, depression and motor activity disturbances, respectively.</span></span><br> <span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span> </strong>Our study indicated that pretreatment of rats with KET, HAL and YOH abolished MPH induced - mood and motor activity disturbances.&nbsp;<br> <strong><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong>MPH by interacting with Dopaminergic, NMDA and ɑ₂-adrenergic receptors induces anxiety, depression and motor activity disorders.</span></span></p> Methylphenidate, Aanxiety, Depression and Motor activity. 309 316 http://journal.zums.ac.ir/browse.php?a_code=A-10-5253-1&slc_lang=en&sid=1 Kowsar Jahanbakshi : Kowsar.Jahabnabkhshi @gmailcom 5200319475328460063957 5200319475328460063957 No Dept. of Medicine, Qom Branch, Islamic Azad University, Qom, Iran Sulail Fatima sulail.foraza@gmail.com 5200319475328460063958 5200319475328460063958 No Dept. of Physiology, Jinnah Medical & Dental College, Sohail University, Karachi, Pakistan Majid Motaghinejad dr.motaghinejad6@gmail.com 5200319475328460063959 5200319475328460063959 Yes Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran Manijeh Motevalian Manijeh.M@gmail.com 5200319475328460063960 5200319475328460063960 No Dept. of Medicine, Qom Branch, Islamic Azad University, Qom, Iran