en Life Science Life Science مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong><em>Plantago psyllium</em> has long been utilised as a medicinal agent worldwide. The current study aims to evaluate the<em> in vivo</em> antimicrobial effects of aqueous <em>P. psyllium </em>and a hydro-alcoholic extract of <em>P. psyllium</em> on <em>Helicobacter pylori</em> as well as the effects of these extracts on liver and kidney in a rat model.<br> <strong><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span> </strong>A total number of 50 female Sprague Dawley rats were experimentally infected (Except for the negative control group). The rats were divided into five groups of 10. The groups were treated by Aqueous and Hydro-Alcoholic <em>P. psyllium</em> leaf extracts. Subsequently, <em>H. pylori</em> antigens which were present in the in rats&rsquo; stool were measured using a serological assay. To diagnose the pathogenicity of the kidney and liver, blood samples which had been taken before and after treatment, were tested for renal and hepatic enzymes by Analyzer Electrolyte and Sysmex KX-21N.<br> <strong><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span> </strong>Following the amoxicillin treatment, the levels of aqueous and hydro-alcoholic extracts of the treated rats were compared with those of the control group, and a significant difference was observed (<em>P</em><0.05). Antigen decrease was observed in all of the groups treated with amoxicillin (<em>P</em>=0.0004), aqueous, and hydro-alcoholic extracts (<em>P</em><0.05). Antigen decrease in the stool was observed in all groups, which indicates that treatment with the herbal extract could affect the infected rats.<br> <strong><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong><em>P. psyllium</em> hydro-alcoholic extract can be applied as a selective treatment for <em>H. pylori</em> infection. Hopefully, experiments suggested that the mentioned extracts could positively affect the process of disease recovery.</span></span></p> Aqueous and Hydro-Alcoholic extracts, Helicobacter pylori, Plantago psyllium 317 323 http://journal.zums.ac.ir/browse.php?a_code=A-10-5288-2&slc_lang=en&sid=1 Shima Keshavarzi shima_keshavarzi89@yahoo.com 5200319475328460065695 5200319475328460065695 No Dept. of Microbiology, Fars Sciences and Research Branch, Islamic Azad University, Shiraz, Iran Masood Sepehrimanesh sepehrimaneshmasood@gmail.com 5200319475328460065696 5200319475328460065696 Yes Dept. of Biology, University of Louisiana at Lafayette, Louisiana, USA Bahman Mirzaei dr.bahman.m@gmail.com 5200319475328460065697 5200319475328460065697 No Dept. of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science, Sari, Iran Omid Koohi-Hosseinabadi koohiomid@yahoo.com 5200319475328460065698 5200319475328460065698 No Central Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran Zeynab Sardari zeinab_sardari@yahoo.com 5200319475328460065699 5200319475328460065699 No Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Mohammad Mehdi Sajjadpur mehdi.sajadpour@yahoo.com 5200319475328460065700 5200319475328460065700 No Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran