en Bionanotechnology Bionanotechnology نامه به سردبیر Letter to the Editor <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><em new="" roman="" style="font-family: " times="">Klebsiella pneumoniae</em><span new="" roman="" times=""> is the most common pathogenic bacterium in the genus </span><em new="" roman="" style="font-family: " times="">Klebsiella </em><span new="" roman="" times="">(1). The aim of this study was to use Polylactic-co-glycolic acid (PLGA) nanoparticles in vaccine design of the capsule antigen of </span><em new="" roman="" style="font-family: " times="">Klebsiella pneumoniae</em><span new="" roman="" times=""> K2O1.</span><br> The capsular antigen was loaded into the polylactic-co-glycolic acid (PLGA) nanoparticles by W/O /W method (5). FT-IR and AFM were used to confirm capsule antigen loading and morphology of the nanoparticles, respectively (6,7). The fever after the vaccination was tested by limolus amoebocyte lysate assay (LAL test)(8). The investigation of the serum of mice by ELISA. Histopathological examination of lung, liver and spleen organs of vaccinated mice in four groups containing five female BALB/C mice (6-7 week-old) was studied after challenge with <em>Klebsiella pneumoniae</em>.&nbsp;Mice vaccinated with nanoparticles containing <em>Klebsiella pneumoniae</em> capsular antigen&nbsp;showed that the proposed vaccine has a high potential for long-term and stable protection against pure <em>Klebsiella pneumoniae</em> capsule antigen, activates (T Helper) lymphocytes T, and stimulates memory by stimulating T cells.&nbsp;Indicating the effectiveness of the vaccine, the results revealed that the vaccine could be recommended for animal studies with more samples or phase one clinical trial studies.</span></span></p> Klebsiella pneumoniae, PLGA, nanoparticles, vaccine,capsular antigen. 73 74 http://journal.zums.ac.ir/browse.php?a_code=A-10-5789-1&slc_lang=en&sid=1 Parivash Ghaderinia parivash_gh1356@yahoo.com 5200319475328460068743 5200319475328460068743 No Dept. of Microbiology, College of Science agriculture and modern technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran Reza Shapouri rezashapoury@yahoo.com 5200319475328460068744 5200319475328460068744 No Dept. of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran Kobra Rostamizadeh rostamizadeh@gmail.com 5200319475328460068745 5200319475328460068745 Yes Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Science, Zanjan, Iran Alireza Khodavandi dr.alirezakhodavandi@gmail.com 5200319475328460068746 5200319475328460068746 No Dept. of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran Mehdi Mahdavi mahdavivac@gmail.com 5200319475328460068747 5200319475328460068747 No Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran