en Pharmacology Pharmacology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong><span style="line-height:200%"><span style="line-height:200%"><i>Rheum turkestanicum</i><b> </b>(<i>R. turkestanicum</i>)<b> </b>has been known to reduce inflammation and has antioxidant properties such as protective effect in neurons. This study aimed to determine the effects of <i>R. turkestanicum</i> on neuronal toxicity induced by the pro-parkinsonian neurotoxin 6-hydroxydopamine (6-OHDA) in neuroblastoma SH-SY5Y cells.</span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span>&nbsp;</strong><span style="line-height:200%"><span style="line-height:200%">MTT and DNA fragmentation by PI staining (sub-G1 peak) assays were used to determine cell viability and induction of apoptosis, respectively. Fluorimetry methods measured lipid peroxidation malondialdehyde (MDA) and reactive oxygen species (ROS) levels. The amount of glutathione (GSH) and the activity of superoxide dismutase (SOD) were measured by DTNB (5, 5&prime;-Dithiobis(2-nitrobenzoic acid)&nbsp; and pyrogallol respectively.</span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span></strong>&nbsp;<span style="line-height:200%"><span style="line-height:200%">Pretreatment with 12.5 to 100 &mu;g/mL of <i>R. turkestanicum</i> extract for 24 hours attenuated 6-OHDA (final concentration 42.5 &mu;g/mL)-induced cytotoxicity. Also, the pretreatment of SH-SY5Y cells with <i>R. turkestanicum </i>inhibited 6-OHDA-stimulated apoptosis in a dose-dependent manner. Additionally, <i>R. turkestanicum</i><b> </b>extract<b> </b>repressed 6-OHDA-induced oxidative stress as measured by the MDA, ROS, GSH, and SOD levels.</span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong><span style="line-height:200%"><span style="line-height:200%">The findings suggest that <i>R. turkestanicum</i><b> </b>extract<b> </b>has neuroprotective activity on 6-OHDA-induced neuronal toxicity of neuroblastoma cells.</span></span></span></span></p> Rheum turkestanicum, Oxidative stress, 6-Hydroxydopamine, Neuroblastoma, Apoptosis 553 560 http://journal.zums.ac.ir/browse.php?a_code=A-10-5957-1&slc_lang=en&sid=1 Roghayeh Rashidi rashidir931@mums.ac.ir 5200319475328460073699 5200319475328460073699 No Dept. of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran Amir Reza Afshari AmirReza.Afshari2@gmail.com 5200319475328460073700 5200319475328460073700 No Dept. of Physiology and Pharmacology, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnourd, Iran Hamid Mollazadeh mollazadehh901@mums.ac.ir 5200319475328460073701 5200319475328460073701 No Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran Azar Hosseini HosseiniAZ@mums.ac.ir 5200319475328460073702 5200319475328460073702 Yes Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran