en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong><span style="line-height:150%"><span style="line-height:150%">Obesity is a major public health problem whose prevalence has constantly increased worldwide. Obesity is a risk factor for several diseases such as type 2 diabetes, fatty liver disease, hyperlipidemia, cardiovascular diseases, and cancer, all of which reduce the quality of life and life expectancy. This study <span style="background:white">investigated</span> anti-adipogenic effects of <i><span style="background:white">Eryngium billardierei</span></i><span style="background:white"> (Ery) extract.</span></span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span>&nbsp;</strong><span style="line-height:150%"><span style="line-height:150%">The <span style="background:white">nontoxic concentration of Ery extract was estimated via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliu bromide (MTT) assay. </span>The differentiation of mouse-derived 3T3-L1 preadipocytes into mature adipocytes was induced in the presence or absence of <span style="background:white">Ery extract. Then, the accumulation of </span></span><span style="background:white"><span style="line-height:150%">lipid droplets was identified by Oil Red O staining. </span></span><span style="line-height:150%">Triacylglycerol level and </span><span style="line-height:150%">glycerol-3phosphate dehydrogenase (GPDH) activity were also measured.</span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span></strong>&nbsp;<span style="line-height:150%"><span style="line-height:150%">The MTT assay showed that 3 mg/mL of the Ery extract can be a nontoxic dose for other analyses. Oil Red O staining demonstrated a reduction of </span><span style="line-height:150%">lipid droplets in the extract-treated adipocyte 3T3-L1 compared to the non-treated group. A significant decrease of triacylglycerol level in the treated 3T3-L1 adipocyte (0.32&plusmn;0.03 mgTG/mg protein) compared with the nontreated group (0.78&plusmn;0.05 mgTG/mg protein) (P<0.01), and a significant decrease of glycerol-3-phosphate dehydrogenase (GPDH) activity in the 3T3-L1adipocyte treated (15.3&plusmn;2.1 U/ml) compared to the non-treated group (30.2&plusmn;3.9 U/ml) (P<0.05) confirmed the </span><span style="line-height:150%">inhibitory action of <span style="background:white">Ery</span> extract on adipocyte differentiation.</span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong><span style="line-height:150%"><span style="line-height:150%">Ery<span style="background:white"> extract, as a safe herbal extract, has </span>anti-adipogenic activity and merits more investigation as a candidate for <span style="background:white">developing an </span></span><span style="background:white"><span style="line-height:150%">anti-obesity drug or supplement.</span></span></span></span></span></p> Adipocyte Differentiation, Eryngium billardierei, GPDH Activity, 3T3-L1 Cell Line 391 397 http://journal.zums.ac.ir/browse.php?a_code=A-10-6338-1&slc_lang=en&sid=1 Ali Heidari aliheydari32610@gmail.com 5200319475328460077765 5200319475328460077765 No Dept. of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Gholamhosein Meftahi meftahi208@yahoo.com 5200319475328460077766 5200319475328460077766 No Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Farideh Bahrami Farideh_bahrami@yahoo.com Farideh_bahrami@yahoo.com 5200319475328460077767 5200319475328460077767 No Dept. of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Zahra Bahari bahari_441@yahoo.com 5200319475328460077768 5200319475328460077768 No Dept. of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Ali Zarei Mahmoudabadi zareiali4031@yahoo.com 5200319475328460077769 5200319475328460077769 No Dept. of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Hasan Fallah Huseini huseini_fallah@yahoo.com 5200319475328460077770 5200319475328460077770 No Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Zohreh Jangravi Jangraviz89@gmail.com 5200319475328460077771 5200319475328460077771 Yes Dept. of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran