en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Background and Objective:</span></span>&nbsp;</strong><span style="line-height:150%"><span style="line-height:150%">Microsatellites are ideal markers for detecting population differences in humans and </span>considered as potentially <span style="line-height:150%">a useful tool</span> and biomarkers<span style="line-height:150%"> in forensic medicine. This study </span>aimed to <span style="line-height:150%">examine and compare</span> the diagnostic value of three<span style="line-height:150%"> Y-</span>STRs <span style="line-height:150%">loci in racial studies</span> by HRM method.</span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span>&nbsp;</strong><span style="line-height:150%"><span style="line-height:150%">Peripheral blood samples </span>of <span style="line-height:150%">200 Iranian Kurdish men </span>living in <span style="line-height:150%">western </span>cities <span style="line-height:150%">of Iran </span>(Kermanshah, Sanandaj, Sardasht, and Ilam) <span style="line-height:150%">were </span>collected and <span style="line-height:150%">analyzed</span> <span style="line-height:150%">for allel</span>e and haplotype <span style="line-height:150%">frequen</span>cies using HRM technique during 2017 to 2019.</span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span></strong>&nbsp;<span style="line-height:150%"><span style="line-height:150%">Most allel</span>ic<span style="line-height:150%"> replications in the AC004617 (І), AC004617 (ІІ) and AC022486 loci were </span>related to<span style="line-height:150%"> alleles </span>13<span style="line-height:150%">, </span>29 and 30<span style="line-height:150%">, and 12, respectively. Also, the AC022486 locus was potentially more beneficial as a population differentiation</span> marker than <span style="line-height:150%">the </span>other <span style="line-height:150%">three </span>studied <span style="line-height:150%">loci.</span></span><br> <strong new="" roman="" style="font-family: " times=""><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span>&nbsp;</strong><span style="line-height:107%">T<span style="line-height:107%">he HRM technique </span>was <span style="line-height:107%">an accurate and inexpensive method</span> for investigating the genetic differences between the four studied populations.</span></span></span></p> Y-STR, HRM, Kurdish Men, AC004617, AC022486 472 480 http://journal.zums.ac.ir/browse.php?a_code=A-10-2271-4&slc_lang=en&sid=1 Nooshin Hashami Nia nooshin_biotech@yahoo.com 5200319475328460079022 5200319475328460079022 No Dept. of Genetics, Faculty of Advance Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran Farouq Karimpour f2015.karimpour@gmail.com 5200319475328460079023 5200319475328460079023 No Iranian Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran Fatemeh Keshavarzi gol.keshavarzi@gmail.com 5200319475328460079024 5200319475328460079024 Yes Dept. of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran