en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="line-height:150%"><b><span style="line-height:150%">Background & Objective: </span></b><span style="line-height:150%">Acute kidney injury (AKI) is a rapid loss of kidney function that is associated with high morbidity and mortality. Oxidative hazard, inflammation, mitochondrial deterioration and depletion of cellular energy stores, which terminate in organ dysfunction, are the major hallmarks of AKI. The current experimental investigation attempted to evaluate the effects of selenium (Se), a pivotal micronutrient, on the ischemia/reperfusion (IR)-induced kidney damage emphasizing on the biogenesis of mitochondria.</span></span><br> <span style="line-height:150%"><b><span style="line-height:150%">Materials & Methods: </span></b><span style="line-height:150%">Male Wistar rats (n&nbsp;= 18) were randomly allocated into three groups: sham, IR, and Se + IR. Rats in the last group 1 h before IR induction, were treated with Se (0.5 mg/kg) intraperitoneally. Six hours after reperfusion blood and kidney tissue samples were collected, and animals were euthanized. In addition to the evaluation of biochemical factors and histopathology, the protein levels of sirtuin1 (SIRT-1), and peroxisome proliferator-activated receptor-gamma coactivator 1-&alpha; (PGC-1&alpha;) of the kidney tissues were determined via western blotting.</span></span><br> <span style="line-height:150%"><b><span style="line-height:150%">Results: </span></b><span style="line-height:150%">Pre-treatment with Se could significantly improve IR-induced kidney function markers (creatinine and BUN) as well as the pathological alteration in comparison with the IR group (P&nbsp;< 0.05). Moreover, in the Se + IR group, a substantial surge of the Sirt-1 and PGC-1&alpha; at the protein level was recorded compared to the IR group.</span></span><br> <span style="line-height:150%"><b><span style="line-height:150%">Conclusion: </span></b><span style="line-height:150%">The results proposed that Se displays a protective role against renal IR injury via up-regulating proteins involved in mitochondrial biogenesis. Due to the pivotal role of mitochondria in renal tubules, these results offer insight into the plausible preventative and/or therapeutic effects of&nbsp;Se against AKI after further studies.</span></span></span></span></p> Selenium, Ischemia, Reperfusion Injury, Mitochondria, Sirtuins 41 47 http://journal.zums.ac.ir/browse.php?a_code=A-10-6308-2&slc_lang=en&sid=1 Amin Abdollahzade Fard af.amin@yahoo.com 5200319475328460080457 0009-0000-3495-373x No Nephrology and Kidney Transplant Research Center, Clinical Research Institute, Urmia University of Medical Sciences, Urmia, Iran Leila Chodari lchodari@yahoo.com 5200319475328460080458 5200319475328460080458 No Dept. of Physiology, School of medicine, Urmia University of Medical Sciences, Urmia, Iran Telli Alizade Telalizade@gmail.com 5200319475328460080459 0009-0002-4988-3660 No Dept. of Pharmaceutical Technology and Management, Faculty of Pharmacy, Azerbaijan Medical University, Baku, Azerbaijan Farah Madatli madfarah3003@gmail.com 5200319475328460080460 0009-0000-3495-373x No Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Elham Ahmadian ahmadian.elham@yahoo.com 5200319475328460080461 5200319475328460080461 Yes Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Fariba Mahmoodpoor mahmoodpoorf@yahoo.com 5200319475328460080462 5200319475328460080462 No Research Center For Integrative Medicine in Aging, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran