en Bionanotechnology Bionanotechnology مقاله پژوهشی Original Research Article <span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="background:#2d7f8f"><span style="color:white">Background & Objective:</span></span></span></b><b> </b><b>&nbsp;</b><span style="font-size:9.0pt"><span style="color:black">Antibiotic resistance has become a global problem. This study aimed to develop and evaluate nanoemulsions&#39; capacity to improve Cuminum cyminum&#39;s antibacterial activity essential oil as an appropriate approach to prevent microbial resistance.</span></span></span></span><br> <span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="background:#2d7f8f"><span style="color:white"><span style="letter-spacing:-.1pt">&nbsp;Materials & Methods:</span></span></span></span></b> &nbsp;<span style="font-size:9.0pt"><span style="color:black"><span style="letter-spacing:-.1pt">Gas chromatography-mass spectrometry was used to determine the main constituent of C. cyminum essential oil. FT-IR was used to investigate the functional groups of chemical composition. A nanoemulsion was generated using high-shear homogenization, followed by several physicochemical characterizations. The antibacterial activity of essential oil in both pure and nanoemulsion forms against important food-borne pathogens, and drug-resistant bacteria was determined by measuring the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC).</span></span></span></span></span><br> <span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span lang="EN-IN" style="font-size:9.0pt"><span style="background:#2d7f8f"><span style="color:white">Results: </span></span></span></b><b>&nbsp;</b><span style="font-size:9.0pt"><span style="color:black"><span style="letter-spacing:-.1pt">Cumin aldehyde with 20.08%w/v is the main constituent of essential oil. The prepared nanoemulsions had an average size of 72 nm, particle distribution of 0.234, and zeta potential of 26 mV. Nanoemulsion formulations of essential oils are more effective compared to pure ones.&nbsp;&nbsp; <i>Klebsiella pneumonia</i> and <i>Pseudomonas aeruginosa </i>have the highest and the lowest antimicrobial effects of essential oil among all studied bacteria, respectively. In contrast, in resistant samples, the major and minor effects were contributed to Methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus, respectively.</span></span></span></span></span><br> <span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="background:#2d7f8f"><span style="color:white">Conclusion: </span></span></span></b><b>&nbsp;</b><span style="font-size:9.0pt"><span style="color:black">Cumin oil-nanoemulsion had an acceptable function against antibiotic-resistant bacteria. It is advisable to combine this formulation with synthetic antibiotics in order to reduce bacterial resistance and serve as a preservative in the pharmaceutical and food industries. </span></span></span></span><br> &nbsp; Cuminum cyminum. L, Essential oil, Characterization, Nanoemulsion, Antibacterial activity. 427 437 http://journal.zums.ac.ir/browse.php?a_code=A-10-106-4&slc_lang=en&sid=1 Fatemeh Hosseinali Khani fatemehosseinalikhani@gmail.com 5200319475328460082295 5200319475328460082295 No Zanjan university of Medical ScienceU Alireza Yazdinezhad yazdinezhad@zums.ac.ir 5200319475328460082296 5200319475328460082296 No Zanjan University of Medical Sciences Somayeh Sadighian sadighian@zums.ac.ir 5200319475328460082297 5200319475328460082297 No Zanjan University of Medical Sciences mehran mohseni mohsenim@zums.ac.ir 5200319475328460082298 5200319475328460082298 Yes Zanjan University of Medical Sciences