en Health Improvement Strategies Health Improvement Strategies مقاله پژوهشی Original Research Article <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><span new="" roman="" times=""><b><span style="background:#2d7f8f"><span style="color:white">Background & Objective:</span></span></b><b> </b><b>&nbsp;</b>There is considerable evidence in the field of epilepsy research suggesting that melatonin may have a potential therapeutic role in the treatment of epilepsy. Investigating the effects of melatonin on neuronal electrical activity may provide valuable insight into the development of adjunctive therapies in this context. We aimed to investigate the prophylactic properties of melatonin using the intracellular recording technique in an epilepsy model of snail neurons, which exhibit epileptic behavior similar to that in human neurons.</span><br> <span new="" roman="" times=""><b><span style="background:#2d7f8f"><span style="color:white"><span style="letter-spacing:-.1pt">&nbsp;Materials & Methods:</span></span></span></b> To study the impact of melatonin (100 &micro;M) on firing pattern and action potential (AP) configuration in an epileptic condition, the current clamp technique was used on <i>Helix aspersa</i> neurons. Recordings were made before and after administering Pentylenetetrazole (PTZ) (25 mM).</span></span></span><br> <b style="font-family: &quot;Times New Roman&quot;; font-size: 14px; text-align: justify; text-indent: 0in; white-space-collapse: preserve;"><span lang="EN-IN"><span style="background:#2d7f8f"><span new="" roman="" times=""><span style="color:white">Results: </span></span></span></span>&nbsp;</b><span new="" roman="" style="font-family: &quot;Times New Roman&quot;; font-size: 14px; text-align: justify; text-indent: 0in; white-space-collapse: preserve;" times="">The findings demonstrated that applying melatonin to cells in normal Ringer&#39;s solution did not significantly alter the resting membrane potential (RMP) or the amplitude and duration of the AHP and AP. However, a significant decline in frequency was evident (P<0.05).&nbsp; Application of melatonin after PTZ significantly decreased the firing frequency of APs while concurrently enhancing the amplitude of AHP, which had been reduced by PTZ, and hyperpolarizing the RMP.</span><br> <span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="background:white"><span new="" roman="" times=""><b><span style="background:#2d7f8f"><span style="color:white">Conclusion: </span></span></b><span style="color:#262626">Our study demonstrates that melatonin has protective effects against some of the adverse impacts of PTZ on neuronal firing patterns in <i>Helix aspersa</i> neurons. These findings suggest that melatonin may play a crucial role in modulating neuronal excitability, which is important in epilepsy.</span></span></span></span></span></div> Melatonin, Pentylenetetrazole, Intracellular recording, Helix aspersa 378 385 http://journal.zums.ac.ir/browse.php?a_code=A-10-104-5&slc_lang=en&sid=1 Mahin Ganjkhani mganjkhani@zums.ac.ir 5200319475328460086937 5200319475328460086937 Yes Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Marzieh Marahem m.marahem@yahoo.com 5200319475328460086938 5200319475328460086938 No Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran